中国激光, 2020, 47 (2): 0207007, 网络出版: 2020-02-21
整体器官的光透明成像方法综述 下载: 2876次特邀综述
Review of Tissue Optical Clearing Methods for Imaging Whole Organs
生物光学 三维成像 组织光透明 整体器官 光学成像 大组织标记 Bio-optics three-dimensional imaging tissue optical clearing whole organs optical imaging whole-mount immunolabeling
摘要
现代光学成像技术与荧光标记技术不断发展,为高分辨地获取生物组织三维结构信息提供了重要的工具。然而,大多数生物组织具有不透明特性,限制了光在组织中的穿透深度,进而限制了光学成像技术在大组织或整体器官成像中的应用。近年兴起的组织光透明技术通过多种物理、化学手段降低组织对光的衰减,增加光穿透深度,从而提高光学成像的成像深度与成像质量,为整体组织器官的三维成像提供了全新的思路。本文从离体组织光透明方法、大组织器官标记方法、三维整体成像技术三个方面,对整体器官的光透明成像方法进行综述。
Abstract
The technological development in modern optical imaging and fluorescent labeling has provided important tools for obtaining high-resolution three-dimensional (3D) structural information about biological tissues. However, the opaque nature of most biological tissues limits the light penetration depth, which limits their applications for large tissue specimens or organs. In recent years, optical clearing methods that employ various physical and chemical means have been proposed for reducing light attenuation and improving the imaging depth, thereby providing novel perspectives for 3D imaging of large samples or whole organs. This paper reviews the tissue optical clearing methods for imaging whole organs from three aspects: in vitro optical clearing methods for tissues, whole-mount labeling methods, and 3D imaging techniques.
俞婷婷, 朱丹. 整体器官的光透明成像方法综述[J]. 中国激光, 2020, 47(2): 0207007. Yu Tingting, Zhu Dan. Review of Tissue Optical Clearing Methods for Imaging Whole Organs[J]. Chinese Journal of Lasers, 2020, 47(2): 0207007.