Advanced Photonics, 2020, 2 (6): 065002, Published Online: Nov. 30, 2020
High spatial and temporal resolution synthetic aperture phase microscopy Download: 730次
Abstract
A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, while achieving millisecond-level temporal resolution. In HISTR-SAPM, digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability. An off-axis interferometer is used to measure the sample scattered complex fields, which are then processed to reconstruct high-resolution phase images. Using HISTR-SAPM, we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap (i.e., a full pitch of 330 nm). As the reconstruction averages out laser speckle noise while maintaining high temporal resolution, HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells, such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells. We envision that HISTR-SAPM will broadly benefit research in material science and biology.
Cheng Zheng, Di Jin, Yanping He, Hongtao Lin, Juejun Hu, Zahid Yaqoob, Peter T. C. So, Renjie Zhou. High spatial and temporal resolution synthetic aperture phase microscopy[J]. Advanced Photonics, 2020, 2(6): 065002.