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肿瘤血管的高分辨光声定量成像

High-Resolution Photoacoustic Quantitative Imaging of Tumor Vessels

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摘要

利用微米级分辨率的高分辨光声显微成像系统,对小鼠耳部肿瘤早期血管的生长以及肿瘤对抗血管治疗药物的响应过程进行连续无标记的成像监测;提出将基于三维Hessian矩阵的血管提取算法应用于肿瘤血管的光声定量成像,以提高肿瘤血管的提取精度,并定量分析肿瘤血管的直径、密度、弯曲度等形态学信息变化。结果表明,高分辨光声定量成像在肿瘤及其他以血管变化为病理特征的疾病机制研究方面具有巨大的应用潜力。

Abstract

We uses a high-resolution (micrometer scale) photoacoustic microscopic imaging system to continuously monitor early-stage tumor angiogenesis in the ear of mice and its response to anti-angiogenesis therapy. Further, a three-dimensional Hessian-matrix-based vascular extraction algorithm is proposed for the quantitative photoacoustic imaging of tumor vessels to improve the tumor vessel extraction accuracy. Subsequently, the morphological changes in various parameters, such as the diameter, density, and tortuosity, of the tumor vessels are quantitatively analyzed. Furthermore, the potential of high-resolution photoacoustic quantitative imaging to study the pathological mechanisms of tumors and other diseases characterized by vascular changes is demonstrated in this study.

Newport宣传-MKS新实验室计划
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DOI:10.3788/CJL201946.0907001

所属栏目:生物医学光子学与激光医学

基金项目:国家自然科学基金、深圳市科技计划、广州大学科研能力提升计划;

收稿日期:2019-03-20

修改稿日期:2019-05-17

网络出版日期:2019-09-01

作者单位    点击查看

陈宁波:广州大学机械与电气工程学院, 广东 广州 510006中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
周慧超:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055中山大学附属第三医院超声科, 广东 广州 510630
赵煌旋:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
王柏权:广州大学机械与电气工程学院, 广东 广州 510006中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
宋亮:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
刘成波:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
张建辉:广州大学机械与电气工程学院, 广东 广州 510006

联系人作者:刘成波, 张建辉(cb.liu@siat.ac.cn, zhangjh@nuaa.edu.cn)

备注:国家自然科学基金、深圳市科技计划、广州大学科研能力提升计划;

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引用该论文

Ningbo Chen, Huichao Zhou, Huangxuan Zhao, Boquan Wang, Liang Song, Chengbo Liu, Jianhui Zhang. High-Resolution Photoacoustic Quantitative Imaging of Tumor Vessels[J]. Chinese Journal of Lasers, 2019, 46(9): 0907001

陈宁波, 周慧超, 赵煌旋, 王柏权, 宋亮, 刘成波, 张建辉. 肿瘤血管的高分辨光声定量成像[J]. 中国激光, 2019, 46(9): 0907001

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