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基于上转换发光技术的生物传感器及其应用

Up-Converting Phosphor Technology-Based Biosensor and Its Application

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摘要

为实现对特定生物分子的高灵敏度快速检测与分析,采用上转换发光材料作为标记物,研制成功一台基于上转换发光技术的新型光学免疫生物传感器。该传感器利用上转换发光材料在红外光激发下发射可见磷光的特性,通过对免疫层析试纸条上经生物反应而结合上去的上转换发光材料颗粒的含量进行检测,计算出被测样品中特定生物分子的浓度。实验结果表明,该传感器具有较好的生物特异性,对兔抗鼠疫免疫球蛋白(IgG)标准样品的检测灵敏度达到ng/ml量级,并在200~6000 ng/ml浓度范围内具有良好的线性响应特性,相关系数R2≥0.95;对鼠疫耶尔森氏菌抗体的敏感性明显高于间接血凝实验,且与免疫印迹检测实验结果具有较好的一致性。该传感器具有稳定、可靠、灵敏的工作性能,符合实际检测与分析的要求。

Abstract

An up-converting phosphor technology-based biosensor (UPT-based biosensor) was developed for immunoassay and fast detecting using up-converting phosphor (UCP) as the biological marker. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particle on the test strip after immunoreaction, the UPT-based biosensor can obtain the concentration information of target analyte in the sample. The UPT system realized the quantitative detection and had good ability to meet the need of some emergencies. High sensitivity (nanogram/ml), good linear relationship in the concentration range of 200~6000 ng/ml and an excellent correlation (R2≥0.95) were verified by quantitative detection results. The sensitivity of the UPT-based biosensor was better than that of the indirect hemagglutination test in the practical application. All the results were comparable with that obtained by Western Blot detection. The UPT-based biosensor had stable, reliable and sensitive working performances, and it can meet the need of some various applications such as rapid immunoassay, chemical and biological detection and so on.

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中图分类号:TP212.3

所属栏目:医用光学与生物光学

基金项目:国家自然科学基金(60378024)资助课题。

收稿日期:2004-09-13

修改稿日期:2004-11-05

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赵永凯:中国科学院上海光学精密机械研究所, 上海 201800中国科学院研究生院, 北京 100039
周蕾:军事医学科学院微生物流行病研究所, 北京 100074
黄惠杰:中国科学院上海光学精密机械研究所, 上海 201800
杨瑞馥:军事医学科学院微生物流行病研究所, 北京 100074
黄立华:中国科学院上海光学精密机械研究所, 上海 201800
任冰强:中国科学院上海光学精密机械研究所, 上海 201800
卢健:中国科学院上海光学精密机械研究所, 上海 201800中国科学院研究生院, 北京 100039
王向朝:中国科学院上海光学精密机械研究所, 上海 201800

联系人作者:赵永凯(darkskyhaha@siom.ac.cn)

备注:赵永凯(1978~),男,中国科学院上海光学精密机械研究所助理研究员,主要从事光学生物传感器方面的研究。

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