基于光诱导荧光技术的浮游植物光合作用活性原位测量方法

根据浮游植物在不同光照下的荧光诱导特性, 研究了叶绿素荧光作为浮游植物光合作用探针的特点, 提出了原位测量活体叶绿素荧光值Ft和Fm获取浮游植物光合作用活性的方法。以淡水湖泊浮游植物中的普通小球藻、铜绿微囊藻和梅尼小环藻为实验对象, 将原位测量方法得到的数据和实验室水样荧光仪的测试结果进行对比分析, 结果表明: 普通小球藻、梅尼小环藻和铜绿微囊藻的两组测试结果之间的相关系数分别为0.977 8, 0.867 8和0.776 8, 研究为进一步实现水体浮游植物光合作用活性的快速连续原位测量提供了方法。

Abstract

According to the phytoplankton fluorescence induction characteristics under different light conditions, chlorophyll fluorescence as a probe for analysis of phytoplankton photosynthesis was studied. The present paper proposed a in-situ measurement method based on the chlorophyll fluorescence values Ft and Fm to get phytoplankton photosynthesis activity, Chlorella vulgaris, microcystis aeruginosa and Cyclotella meneghiniana Kiits were selected as experimental subjects, a comparison test was done between self-developed in-situ measurement system and Water PAM in lab, and The results showed that coefficients between the two methods were 0.977 8, 0.878 6 and 0.795 3. This work provides a rapid and in-situ measurement method for phytoplankton photosynthesis activity.

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刘晶, 刘文清, 赵南京, 张玉钧, 马明俊, 殷高方, 戴庞达, 王志刚, 王春龙, 段静波, 余晓娅, 方丽. 基于光诱导荧光技术的浮游植物光合作用活性原位测量方法[J]. 光谱学与光谱分析, 2013, 33(9): 2443. LIU Jing, LIU Wen-qing, ZHAO Nan-jing, ZHANG Yu-jun, MA Ming-jun, YIN Gao-fang, DAI Pang-da, WANG Zhi-gang, WANG Chun-long, DUAN Jing-bo2, YU Xiao-ya, FANG Li. The Method of Phytoplankton Photosynthesis Activity In-Situ Measurement Based on Light Induced Fluorescence[J]. Spectroscopy and Spectral Analysis, 2013, 33(9): 2443.

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