电感耦合等离子体串联质谱法测定野生皂荚中多种微量元素

采用电感耦合等离子体串联质谱(ICP-MS/MS)法测定野生皂荚中多种微量元素的含量。皂荚样品以HNO3+H2O2+HCl为混合消解试剂经微波消解后直接测定。在MS/MS模式下向碰撞反应池中通过氧气或氨气, 分别利用氧气质量转移、氧气原位质量和氨气质量转移消除多原子离子质谱干扰。通过分析国家标准参考物质绿茶(GBW10052)和芹菜(GBW10048)验证方法的准确性和精密度, 所测定结果与参考值基本一致, RSD≤7.8%。采用所建立的方法分别分析了来自中国云南和重庆武陵山区野生皂荚中的12种微量元素(V, Cr, Co, Cu, Zn, Fe, Mn, Ni, As, Cd, Hg, Pb), 12种微量元素的检出限为0.29～21.36 ng·L-1。结果显示, 野生皂荚12种分析元素中Fe含量最高, 其次为元素Zn, 重金属元素As, Cd, Hg和Pb的含量远低于《中国药典》限量标准。该研究为野生皂荚中微量元素的安全评价和监管提供了科学方法和数据。

Abstract

An analysis method was developed to detect trace elements in Chinese Honeylocust Fruit by using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). All samples were microwave digested by using (HNO3+H2O2+HCl). In the MS/MS mode, the polyatomic interferences were eliminated by the utilization of O2 mass shift, O2 on-mass, and NH3 mass shift with O2 or NH3 in the collision/reaction cell of the ICP-MS/MS. The analytical results of proposed method were validated by analysis of the national standard reference samples of green tea (GBW10052) and celery (GBW10048), which showed good agreement with the certified values, RSD≤7.8%. Furthermore, 12 trace elements (V, Cr, Co, Cu, Zn, Fe, Mn, Ni, As, Cd, Hg, and Pb) in these samples collected from Yunnan and Chongqing Wuling Mountain, were detected by proposed analyzed method. The detection limits of the analytes were in the range of 0.29～21.36 ng·L-1. The analytical results showed that Fe and Zn elements were rich in these wild samples, while heavy elements in these samples were lower than limitation standard of Chinese pharmacopoeia. This study can be used to estimate the trace element and quality of wild honey locust. Also, this study can provide scientific methods and data for the safety assessment and regulation of trace elements in Chinese Honeylocust Fruit.

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江波, 黄建华, 刘伟. 电感耦合等离子体串联质谱法测定野生皂荚中多种微量元素[J]. 光谱学与光谱分析, 2017, 37(12): 3859. JIANG Bo, HUANG Jian-hua, LIU Wei. Multi-Element Analysis of Wild Chinese Honeylocust Fruit by Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS/MS)[J]. Spectroscopy and Spectral Analysis, 2017, 37(12): 3859.

电感耦合等离子体串联质谱法测定野生皂荚中多种微量元素

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