光学学报, 2001, 21 (4): 509, 网络出版: 2006-08-10  

活体声荧光成像研究

Study on Sonoluminescence Imaging of Living Body
作者单位
华南师范大学激光生命科学研究所,广州 510631
摘要
声荧光应用于生物组织成像研究是最近二年发展起来的新兴领域,本文报道利用高灵敏度的致冷CCD探测系统获取了活体声荧光图像,同时利用一种能在活体内增强声荧光的化学发光试剂FCLA(Fluoresceinyl Cypridina Luminescent Analog,海荧荧光素类似物),成功地实现了动物在体成像。这种方法可望在医学影像诊断中得到广泛的实际应用。
Abstract
Imaging biological tissues using sonoluminescence (SL) is a new research field developing in the latest two years. Sonoluminescence image of living body (nude mouse) was obtained by the high quantum efficiency imaging system. Fluoresceinyl Cypridina Luminescent Analog (FCLA), a chemiluminescent analytic agent, is used in vivo for the first time to enhance biological SL. An image of FCLA enhanced SL in vivo was observed too. This technique has potential application in clinics for imaging diagnosis.
参考文献

[1] Frenzel H, Schultes H. Luminescenz im ultraschallbeschikten Wasser. Z. Phys. Chem. Abt., 1934, 27B(6):421~424

[2] Moss W C, Clarke D B, Young D A. Calculated pulse widths and spectra of a single sonoluminescing bubble. Science, 1997, 276(5317):1398~1401

[3] Hilgenfeldt S, Grossmann S, Lohse D. A simple explanation of light emission in sonoluminescence. Nature, 1999, 398(6726):402~405

[4] Wang L V, Shen Q. Sonoluminescent tomography of strong scattering media. Opt. Lett., 1998, 23(7):561~563

[5] Shen Q, Wang L V. Two-dimensional imaging of dense tissue-simulating turbid media by use of Sonoluminescence. Appl. Opt., 1999, 38(1):246~252

[6] Crum L A. Sonoluminescence. Physics Today, 1994, 47(9):22~29

[7] Nakano M, Sugioka K, Ushuima Y et al.. Chemiluminescence probe with cypridina luciferin analog estimating the ability of human granulocytes to generate O-2. Analytical Biochemistry, 1996, 159(4):363~369

[8] 王维江,邢达,谭石慈等.生物超弱发光图像的探测与分析. 生物物理学报, 1997,13(4):677~682

[9] 谭石慈,邢达.生物超微弱发光图像的观测. 科学通报,1997,42(6):643~645

[10] 邢达,唐永红,谭石慈等.植物体应激反应中生物光子发射的实验观测. 科学通报,1999,44(21):2299~2302

[11] 陈季武,胡天喜.一种新的测量单线态氧的化学发光体系. 发光学报,,1995,16(3):273~277

[12] 胡天喜. 测量活性氧、自由基及脂质过氧化物的化学发光技术. 自由基生命科学进展,第5集,北京:原子能出版社, 1997. 65~73

[13] 邢达,于彦华,周俊初等.利用激光共聚焦扫描显微镜的GFP荧光成像. 光学学报,1999,19(10):1439~1440

[14] He Yonghong, Xing Da, Tang Yonghong et al.. FCLA-enhanced sonoluminescence imaging in vivo. Proc. SPIE, 1999, 3863:129~133

何永红, 邢达, 唐永红, 谭石慈. 活体声荧光成像研究[J]. 光学学报, 2001, 21(4): 509. 何永红, 邢达, 唐永红, 谭石慈. Study on Sonoluminescence Imaging of Living Body[J]. Acta Optica Sinica, 2001, 21(4): 509.

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