光学学报, 2010, 30 (12): 3622, 网络出版: 2010-12-07   

基于液晶光谱仪的西洋参品质荧光检测方法

Panax Quinquefolium′s Active Ingredients Detecting Method Based on LCTF Spectrograph
作者单位
1 暨南大学广东省高等学校光电信息与传感技术重点实验室, 广东 广州 510630
2 南方医科大学中药学院, 广东 广州 510515
3 华南农业大学应用物理系, 广东 广州 510640
摘要
利用基于可调液晶滤光器做为分光器件的液晶光谱仪,对西洋参进行了紫外光照射下的连续荧光光谱成像检测。检测中发现西洋参切片存在着显著的荧光特性,通过对不同等级的西洋参及西洋参的有效成份(人参总皂苷)进行分类检测,验证西洋参所包含的荧光物质为人参总皂苷,并且所发出的荧光强度与其总皂苷含量存在对应关系,因此可以通过检测西洋参的荧光光谱强度判断其品质。从而,得到一种简单、快速和无损的西洋参品质判定和成份检测的方法。同时,通过对西洋参切片荧光光谱图像的分析处理得到了西洋参的荧光光谱曲线和总皂苷分布的三维图谱,获得了西洋参总皂苷含量的三维空间分布信息。
Abstract
A liquid crystal spectrograph is designed to collect Panax quinguefolium′s spectral image based on liquid crystal tunable filter(LCTF)spectroscope. Panax quinquefolium slice is put under UV light, and its fluorescence image is collecteel by the instrument continuously. Panax quinguefolium′s fluorescence effect is obvious during the detection. Corresponding relations are found between fluorescence intensity and saponins which is panax quinquefolium′s effective active ingredient, when the slice of different grades and standards is detected. So a simple, fast, non-destructive method, which can be applied to assess panax quinquefolium′s quality and ingredient, is established. More over, the slice′s fluorescence curve of spectrum and panax quinquefolium′s saponins 3 dimensional distribution spectrum can be obtained, its saponins′s distribution information can also be obtained.
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李子雷, 王琳, 庞其昌, 马骥, 赵静, 林富斌. 基于液晶光谱仪的西洋参品质荧光检测方法[J]. 光学学报, 2010, 30(12): 3622. Li Zilei, Wang Lin, Pang Qichang, Ma Ji, Zhao Jing, Lin Fubin. Panax Quinquefolium′s Active Ingredients Detecting Method Based on LCTF Spectrograph[J]. Acta Optica Sinica, 2010, 30(12): 3622.

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