红外与激光工程, 2019, 48 (8): 0819001, 网络出版: 2019-09-03   

太赫兹技术的液体中同型半胱氨酸的定量识别研究

Quantitative identification of homocysteine in liquid based on terahertz technique
作者单位
上海理工大学 光电信息与计算机工程学院, 上海200093
摘要
同型半胱氨酸是一种含硫氨基酸, 为蛋氨酸和半胱氨酸代谢过程中产生的重要中间产物, 它在人体血液或尿液中浓度含量过高时会大幅增加心血管疾病、老年痴呆、骨折等疾病的发病风险。基于太赫兹时域光谱系统, 针对液体状态下同型半胱氨酸的检测提出了浓缩富集再烘干的处理方法, 有效测量了不同浓度下同型半胱氨酸的太赫兹吸收光谱。经过与拉曼光谱方法的结果比对, 证明太赫兹检测的精度比拉曼光谱检测的精度提升了3倍。该结果对临床医学中同型半胱氨酸相关疾病的准确、快速诊断具有重要意义。
Abstract
Homocysteine is a sulfur-containing amino acid, which is an important intermediate product produced during the metabolism of methionine and cysteine. High concentration of homocysteine in human blood or urine will significantly increase the risk of cardiovascular disease, alzheimer′s disease, bone fracture and other diseases. Based on terahertz time domain spectroscopy (THz-TDS), a method of concentration, enrichment and drying was proposed for the detection of homocysteine in liquid state. The THz absorption spectra of homocysteine at different concentrations were measured effectively. Compared with the results of Raman spectroscopy, the accuracy of terahertz detection was three times higher than that of Raman spectroscopy. The results were of great significance for the accurate and rapid diagnosis of homocysteine-related diseases in clinical medicine.
参考文献

[1] Mudd S H, Finkelstein J D, Refsum H, et al. Homocysteine and its disulfide derivatives: A suggested consensus terminology [J]. Arteriosclerosis Thrombosis and Vascular Biology, 2000, 20(7): 1704-1706.

[2] Jacques P F, Selhub J, Bostom A G, et al. The effect of folic acid fortification on plasma folate and total homocysteine concentrations [J]. New England Journal of Medicine, 1999, 340(19): 1449-1454.

[3] Refsum H, Helland S, Ueland P M. Radioenzymic determination of homocysteine in plasma and urine [J]. Clinical Chemistry,1985, 4(31): 624-628.

[4] Liu P, Huang J, Zhong L W. Role and mechanism of homocysteine in affecting hepatic protein-tyrosine phosphatase 1B[J]. BBA-General Subjects, 2019, 1863(5): 941-949.

[5] Kubota Y, Alonso A, Heckbert S R, et al. Homocysteine and incident atrial fibrillation: the atherosclerosis risk in communities study and the multi-ethnic study of atherosclerosis[J]. Heart, Lung and Circulation, 2019, 28(4): 615-622.

[6] Zhong H, Redo-Sanchez A, Zhang X C . Identification and classification of chemicals using terahertz reflective spectroscopic focal-plane imaging system [J]. Biomedical Optics Express, 2006, 14(20): 9130-9141.

[7] Ueno Y, Rungsawang R, Tomita I, et al. Quantitative measurements of amino acids by terahertz time-domain transmission spectroscopy[J]. Analytical Chemistry,2006, 78(15): 5224-5228.

[8] Zeitler J A, Newnham D A, Taday P F, et al. Temperature dependent terahertz pulsed spectroscopy of carbamazepine [J]. Thermochemical ACTA, 2005, 436(1-2): 71-77.

[9] Ajito K, Ueno Y. THz chemical imaging for biological applications [J]. IEEE Transactions on Terahertz Science and Technology, 2011, 1(1): 290-300.

[10] Peng Y, Shi C, Xu M, et al. Qualitative and quantitative identification of components in mixture by terahertz spectroscopy[J]. IEEE Transactions on Terahertz Science and Technology, 2118, 8(6): 696-671.

[11] Seshadri S, Beiser A, Selhub J, et al. Plasma homocysteine as a risk factor for dementia and alzheimer′s disease [J]. New England Journal of Medicine, 2002, 346(7): 476-483.

[12] Zheng X M, McLaughlin C V, Cunningham P, et al. Origin broadband terahertz sources and sensors[J]. Journal of Nanoelectronics and Optoelectronics, 2007, 2(1): 58-76.

[13] Peng Y, Yuan X, Zou X, et al. Terahertz identification and quantification of neurotransmitter and neurotrophy mixture[J]. Biomedical Optics Express, 2016, 7: 4472-4479.

[14] Chen W Q, Peng Y, Jiang X K, et al. Isomers identification of 2-hydroxyglutarate acid disodium salt (2 HG) by terahertz time-domain spectroscopy [J]. Scientific Reports, 2017, 7(1): 12166.

[15] Ueno Y, Rungsawang R, Tomita I, et al. Quantitative measurements of amino acids by terahertz time-domain transmission spectroscopy[J]. Analytical Chemistry,2006, 78(15): 5224-5228.

[16] Gunasekarana S, Brightb A, Renuga Devib T S, et al. Experimental and semi-empirical computations of the vibrational spectra of methionine, homocysteine and cysteine [J]. Archives of Physics Research, 2010, 1(1):12-26.

严俊, 汪丽平, 李恬, 王莹莹, 彭滟. 太赫兹技术的液体中同型半胱氨酸的定量识别研究[J]. 红外与激光工程, 2019, 48(8): 0819001. Yan Jun, Wang Liping, Li Tian, Wang Yingying, Peng Yan. Quantitative identification of homocysteine in liquid based on terahertz technique[J]. Infrared and Laser Engineering, 2019, 48(8): 0819001.

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