He-Ne激光照射巨噬细胞对胞内钙浓度及其免疫活性的影响

用He-Ne激光辐照巨噬细胞,实时观测活体单细胞内钙浓度([Ca2+]i)分布和细胞免疫活性随激光功率和照射时间的变化.实验结果表明,随激光照射时间的增加,[Ca2+]i出现上升达到最大值后又下降,逐渐回复初始状态,且呈现中心最强,径向衰减的环形梯度分布.不同激光剂量对巨噬细胞内[Ca2+]i及其免疫活性有着不同的影响,同一波长激光,当剂量不同时,可以表现为完全相反的效应.同时激光功率也是影响巨噬细胞内[Ca2+]i及其免疫活性的一个重要因素,同一波长、同一剂量情况下,如果激光功率不同,巨噬细胞内[Ca2+]i及其免疫活性的变化也有着显著的差异.当照射功率为0.16 mW时,胞内[Ca2+]i峰值是照射功率为0.40 mW时的近6倍.最大值时对应细胞免疫活性最高.对其机理作了初步探讨.

Abstract

Using He-Ne laser to irradiate macrophages, the _i distribution of the single macrophage and the immunocompetence are studied in real-time by change laser power and irradiated time. Experimental result indicated that the _i of the macrophages increases with time and reaches the max value then decreases to preliminary station gradually, presenting cricoid-scalar distribution, the best strong in center and decrease radially. Different laser doses have different impact to macrophages _i and immunocompetence. With same laser wavelength and dose but different laser power, the change of [Ca2+]i and immunocompetence are also different obviously. When laser power is 0.16 mW, the max value of [Ca2+]i is almost six folds higher than that of the laser power is 0.40 mW. The max value of [Ca2+]i corresponding the station of the best strong immunocompetence. The action mechanism is primary discussed in this papaer.

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He-Ne激光照射巨噬细胞对胞内钙浓度及其免疫活性的影响

华南师范大学激光生命科学研究所,广东 广州 510631

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华南师范大学激光生命科学研究所,广东广州 510631