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Spectrophotometer with Enhanced Sensitivity for Uric Acid Detection

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摘要

Spectrophotometer with LED being the light source for uric acid detection is proposed in this work. The mechanism of the uric acid detection is based on energy absorbed by sodium urate, which is a chemical product of uric acid and sodium hydroxide (NaOH) solution. For the performance validation, comparison between the spectrophotometer with LED and halogen lamp is carried out. Measurement results suggest that spectrophotometer system with LED light has better sensitivity than that with halogen light. At 460 nm wavelength, the sensitivity for the spectrophotometer with LED is 0.0046 dL/mg, which is 73% higher than that with halogen light that records 0.0012 dL/mg. This enhanced sensitivity is attributed to the higher luminous efficacy of the LED light beam. As a result, a larger amount of flux interacts with the sample, leading to the sensitivity enhancement. The spectrophotometer with LED is also applied for detection of uric acid in real human urine sample. Based on the experimental data at 460 nm wavelength, the method manages to achieve the sensitivity of 0.0016 dL/mg, accuracy of 96.01%, limit of detection (LOD) of 4.79 mg/dL and limit of quantification (LOQ) of 14.52 mg/dL. These findings show that the use of LED as the input light is promising for the spectrophotometer.

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作者单位:

    Universiti Teknologi Malaysia
    Universiti Tun Hussein Onn Malaysia
    Universiti Teknologi Malaysia
    Universiti Teknologi Malaysia
    Universiti Teknologi Malaysia
    Universiti Tun Hussein Onn Malaysia Fakulti Kejuruteraan Elektrik dan Elektronik
    SIRIM Berhad

引用该论文

Che Abd Rashid Nurfatihah,Ngajikin Nor Hafizah,Azmi Asrul Izam,Arsat Rashidah ,Isaak Suhaila,Cholan Noran Azizan,Azmi Nur Ellina. Spectrophotometer with Enhanced Sensitivity for Uric Acid Detection[J].Chinese Optics Letters,2019,17(8):08.