光学学报, 2017, 37 (11): 1118001, 网络出版: 2018-09-07
基于电光调制器的高速偏振荧光显微系统 下载: 823次
Fluorescence Microscopy System with High Speed Polarization Based on Electro Optical Modulator
显微 偏振荧光显微 电光调制器 生物分子方向 偏振畸变 microscopy polarized fluorescence microscopy electro optical modulator biomolecular orientation polarization distortion
摘要
为提高偏振荧光图像序列的获取速度,提出利用电光调制器对激发光的偏振方向进行控制并配合相机记录偏振荧光图像的高速偏振荧光显微系统。对激发光路的偏振畸变进行测量和有效补偿,以确保样本平面激发光偏振方向的精确控制。利用巨型单层囊泡样本对实验系统进行测试,结果表明该系统能够以近视频帧率对分子方向信息进行成像,具备监测动态样本分子方向信息的能力。
Abstract
To improve the acquisition speed of polarized fluorescence images, a fluorescence microscopy system with high speed polarization is proposed based on the polarization direction control of exciting light with an electro optical modulator, and polarized fluorescence images are recorded with a camera. The polarization distortion induced by the excitation optical path is measured and precisely compensated to ensure the precision of polarization direction control on sample plane. The experimental system is tested with giant unilamellar vesicles. The results show that the proposed system can effectively map the orientation information of biomolecular at a video frame rate and can be used to monitor orientation information of dynamic sample molecules.
王潇, 赵远, 杨凤, 尹建华. 基于电光调制器的高速偏振荧光显微系统[J]. 光学学报, 2017, 37(11): 1118001. Xiao Wang, Yuan Zhao, Feng Yang, Jianhua Yin. Fluorescence Microscopy System with High Speed Polarization Based on Electro Optical Modulator[J]. Acta Optica Sinica, 2017, 37(11): 1118001.