目前的流式细胞仪有非成像式和成像式两类。非成像式流式细胞仪虽有高吞吐量但无法提供细胞形态学信息;成像式流式细胞仪虽然能得到细胞的形态学信息,但受传统CCD等面阵列成像传感器的帧率限制,吞吐量不高。针对这一问题,提出并设计了一种基于光学相干检测技术的超快流式细胞定量相位成像系统,其有效线扫描成像速度可达100 MHz。细胞成像实验结果表明,设计的实验系统可获取高对比度的细胞定量相位图像以及5万个细胞每秒的理论吞吐量。

The flow cytometry nowadays includes non-imaging-type and imaging-type. The non-imaging flow cytometry has a high throughput, but it cannot provide cell morphological information. The imaging flow cytometry which can obtain cell morphological information has a low throughput due to the frame rate limits of the conventional CCD array imaging sensors. Aiming at this problem, an ultrafast flow cytometry quantitative phase imaging system based on optical coherent detection is proposed and designed in this paper. Its effective line scan imaging speed is up to 100 MHz. The cell imaging experiment results show that the proposed system can acquire high-contrast quantitative cell phase images and a theoretical throughput of 50000 cells per second.