激光与光电子学进展, 2019, 56 (22): 223002, 网络出版: 2019-11-02  

载玻片的激光诱导击穿光谱实验关键参数优化 下载: 791次

Optimizing Key Experimental Parameters for Laser-Induced Breakdown Spectroscopy of Slides
作者单位
河南科技大学物理工程学院, 河南 洛阳 471023
摘要
载玻片是生物和医学领域中放置样本的透明玻璃。为了更好地服务于生产过程中对载玻片组分的实时检测,采用1064 nm激光烧蚀载玻片,采集不同关键实验参数下的等离子体辐射光谱;选取Si I∶288.16 nm、Ca I∶422.67 nm、Na I∶589.00 nm作为分析谱线,以谱线强度和信号稳定性作为指标,对光谱仪采集延时、激光能量、激光重复频率、激光烧蚀方式、透镜到样品的距离等参数进行优化。结果表明:对于激光诱导击穿载玻片实验系统,最优的光谱采集延迟时间为3 μs,最优的激光重复频率为2 Hz,采用单点烧蚀方式可以得到更加稳定的光谱信号;对于焦距为100 mm的聚焦透镜,最佳聚焦位置位于样品表面以下8 mm,即透镜到样品的最佳距离为92 mm;为了避免载玻片被击碎,实验中将单激光脉冲能量设置为150 mJ,得到了谱线强度足够高且信号稳定性也满足要求的光谱信号。通过优化实验系统中的关键参数,为采用激光诱导击穿光谱技术实时、在线、原位检测载玻片的成分和含量提供了实验依据。
Abstract
Slides are transparent pieces of glass used to hold samples in biology and medicine. To improve real-time detection of their content in production environments, slides are ablated using a 1064-nm laser and the plasma's emission spectra are recorded for different experimental parameters. Herein, the Si I 288.16 nm, Ca I 422.67 nm, and Na I 589.00 nm lines are selected for analysis. The spectral intensity and signal stability are used as indices to optimize the key experimental parameters, i.e., spectral acquisition delay time, laser energy and repetition rate, ablation mode, and lens-to-slide distance. Results show that, for the experimental system of laser-induced slide ablation, the optimal spectral acquisition delay time is 3 μs and the optimal laser repetition rate is 2 Hz. A more stable spectral signal can be obtained using a single-point ablation method. In addition, for a lens with a focal length of 100 mm, the optimal focusing position is 8 mm below the slide surface; hence, the optimal lens-to-slide distance is 92 mm. The energy of each laser pulse is set to 150 mJ to avoid damaging the slide while still obtaining spectra with sufficiently highline intensities and signal stabilities to meet the experimental requirements. Optimizing the experimental system's key parameters in this manner provides an experimental basis for real-time, online, in-situ detection of slide using laser-induced breakdown spectroscopy.

常鹏飞, 王静鸽, 仵祎, 李智聪, 朱顺利. 载玻片的激光诱导击穿光谱实验关键参数优化[J]. 激光与光电子学进展, 2019, 56(22): 223002. Pengfei Chang, Jingge Wang, Yi Wu, Zhicong Li, Shunli Zhu. Optimizing Key Experimental Parameters for Laser-Induced Breakdown Spectroscopy of Slides[J]. Laser & Optoelectronics Progress, 2019, 56(22): 223002.

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