中国激光, 2018, 45 (2): 0207024, 网络出版: 2018-02-28
基于激光消融技术的斑马鱼条纹再生的研究 下载: 1020次特邀研究论文
Study of Stripe Regeneration of Zebrafish Based on Laser Ablation Technology
生物光学 斑马鱼 激光消融技术 黄色素细胞 黑色素细胞 biological optics zebrafish laser ablation technology xanthophores melanophores
摘要
使用355 nm纳秒脉冲激光对斑马鱼体表条纹进行消融处理,通过分析色素细胞簇的再生过程研究斑马鱼体表图案的形成及再生机制。结果表明:斑马鱼体表的黄色素细胞簇能够促进周围一定区域内黑色素细胞的再生,该区域限于稍远于紧邻黄色条纹的位置,但在半个条纹宽度以内;这种促进作用具有方向特异性,即黄色素细胞更多地促进其本身条纹纵向处的黑色素细胞再生,但不促进其本身条纹横向处的黑色素细胞再生。这一结果可为理解斑马鱼自主形成周期性条纹提供新的实验支持。
Abstract
In the present work, 355 nm nanosecond pulsed laser is used to ablate zebrafish surface stripes to investigate the mechanism underlying formation and regeneration of pigment cells pattern. The experimental results show that zebrafish surface xanthophore clusters can only promote melanophore regeneration within the distance of half width of a stripe but not close to each other. Meanwhile, xanthophores significantly affect melanophore regeneration in a direction perpendicular to its stripe rather than parallel to its stripe, indicating the orientation specificity. The results can provide new experimental supports for understanding how zebrafish self-organize spatially periodic patterns.
蒋鹏翀, 魏巍, 胡芬, 张心正, 潘雷霆. 基于激光消融技术的斑马鱼条纹再生的研究[J]. 中国激光, 2018, 45(2): 0207024. Jiang Pengchong, Wei Wei, Hu Fen, Zhang Xinzheng, Pan Leiting. Study of Stripe Regeneration of Zebrafish Based on Laser Ablation Technology[J]. Chinese Journal of Lasers, 2018, 45(2): 0207024.