雨生红球藻钙蛋白(caleosin)有稳定油体的功能，为了研究该蛋白的理化特征及功能,利用同源克隆和 cDNA末端快速扩增技术(RACE)相结合的方法获得雨生红球藻 caleosin基因的 cDNA全长序列，并进行生物信息学分析和 HaeClo基因的表达分析。分析表明， HaeClo全长为 1 088 bp，共编码 262个氨基酸，理论等电点为 7.73。BLAST同源比对表明 HaeClo的氨基酸序列与盘藻(Gonium pectoral)和莱茵衣藻(Chlamydomonas rein-hardtii)来源的 caleosin的相似性分别达到 66%和 61%，是一个典型的 caleosin家族成员。多序列比对及系统进化也表明 HaeClo与其他高等植物和低等植物来源的 caleosin有共同的祖先。不同胁迫条件下， HaeClo基因在培养第 3天时表达量均达到最高值，此时高蓝光 +1/4氮处理组表达量达峰值，高白光 +1/4氮处理组表达量次之，且目的蛋白在大肠杆菌中成功表达。本文首次从雨生红球藻中克隆获得编码 caleosin的基因序列，并进行了生物信息学分析，对高光和缺氮不同胁迫条件下的雨生红球藻进行了表达分析，进一步研究了 HaeClo基因在雨生红球藻中的表达，为雨生红球藻中 caleosin的表达和功能研究奠定了基础。

Haematococcus pluvialis caleosin has the function of stabilizing oil bodies. In order to investigate its physical and chemical characteristics and functions, the full-length cDNA sequence of cleosin gene of H. pluvialis was obtained by the method of combining homologous cloning and RACE amplification technology, and by the bioinformatics analysis, HaeClo gene expression analysis was conducted. The analysis showed that HaeClo was 1 088 bp in length, encoding a total of 262 amino acids, and the theoretical isoelectric point of 7.73. BLAST homology comparison revealed that the amino acid sequence of HaeClo had 66% and 61% similarity to caleosin derived from Gonium pectorale and Chlamydomonas reinhardtii, re-spectively, which was a typical caleosin family member. The analysis of multiple alignment and phylogenetic tree showed that caleosin derived from higher plants and lower plants may come from a common ancestor. Under di.erent stress conditions, the expression level of the HaeClo gene reached the highest value on the third day of culture. At this time, the expression level of the high blue light+1/4 nitrogen treatment group reached the peak, and the expression level of the high white light+1/4 nitro-gen treatment group was the second. And the target protein was successfully expressed in E. coli. In this study, the gene se-quence encoding caleosin in H. pluvialis was obtained.rstly, and subjected to bioinformatics analysis, expression analysis of H. pluvialis under di.erent stress conditions of high light and nitrogen de.ciency, and further study of HaeClo gene expres-sion in H. pluvialis,which laid the foundation for the expression and function of caleosin in H. pluvialis.