强激光与粒子束, 2010, 22 (12): 2949, 网络出版: 2011-01-05  

高功率毫米波辐照对秀丽线虫行为学和细胞形态学的影响

Behavioral response and cell morphology changes of Caenorhabditis elegans under high power millimeter wave irradiation
作者单位
1 军事医学科学院 放射与辐射医学研究所, 蛋白质组学国家重点实验室, 北京 100850
2 东南大学 毫米波国家重点实验室, 南京 210096
3 中国科学院 电子学研究所, 北京 100190
摘要
采用角锥喇叭天线, 使用不同输出平均功率的毫米波激光辐照秀丽线虫, 采用实时显微摄像系统记录线虫行为学变化, 并使用体视显微镜观察线虫细胞形态学变化。受照射后自由活动的秀丽线虫能够快速逃离高功率毫米波的辐照区域。当受输出平均功率为10 W和12 W辐照后, 线虫先后出现运动速度增加、运动速度减慢直至身体僵直不动的变化过程。当输出平均功率为5 W时, 线虫出现热耐受现象。细胞形态学观察结果显示:辐照后的线虫腹中卵细胞核结构出现不同程度的异常。高功率毫米波辐照可显著影响线虫的行为学特征及细胞形态学, 同时说明秀丽线虫是可适用于高功率毫米波生物效应研究的模式生物之一。
Abstract
C. elegans were exposed to high power millimeter waves(MMWs) with different mean power densities, to investigate their behavioral response and cell morphology changes under MMW irradiation. The time-course photomicrography system was used to record the behavioral changes of C. elegans. The behavioral response and cell morphology changes were further observed by stereoscopic microscopes. The results show that freely moving C. elegans will escape from the MMW irradiation region quickly. After the exposure to MMWs with output mean power of 10 W and 12 W, the bending speed of C. elegans increases significantly at first, while the movement gradually slows down until the bodies get rigid. However, exposed to 5 W MMW, C. elegans show a distinctive tolerant reaction because of the thermal effect. In addition, cell morphological observations show that the nuclear structure of the eggs are abnormal after MMW irradiation. High power MMW significantly affects the behaviors and cell morphology of C. elegans, which suggests that C. elegans could be used as a typical model species to study the biological effects of MMW irradiation.

任长虹, 袁广江, 高艳, 吴永红, 徐志伟, 徐寿喜, 粟亦农, 刘濮鲲, 张成岗. 高功率毫米波辐照对秀丽线虫行为学和细胞形态学的影响[J]. 强激光与粒子束, 2010, 22(12): 2949. Ren Changhong, Yuan Guangjiang, Gao Yan, Wu Yonghong, Xu Zhiwei, Xu Shouxi, Su Yinong, Liu Pukun, Zhang Chenggang. Behavioral response and cell morphology changes of Caenorhabditis elegans under high power millimeter wave irradiation[J]. High Power Laser and Particle Beams, 2010, 22(12): 2949.

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!