用可见波段、不同波长的Ar+激光激发同一浓度血红蛋白溶液产生荧光光谱的研究结果表明,血红蛋白在628 nm附近存在一个较强的荧光谱峰,且随激励激光波长的红移其相对光强依次增大,从而解释了临床上用He-Ne激光治疗疗效显著的物理机理。理论研究结果认为该血红蛋白的荧光光谱主要是血红蛋白中存在的卟啉类荧光团的贡献。还利用475.6 nm的激光分别激发浓度为1%～7%的血红蛋白溶液,根据所获得的荧光光谱发现,其谱峰位置几乎不随样品浓度的改变而改变,该结果显示了激光与普通光对生物大分子存在明显不同的作用特性。

Based on argon laser of different wavelengths in visible light excited hemoglobin fluorescence spectra, it shows that there is one prominent fluorescence peak near 628 nm and the intensity increases with excitation light red shifting. The theoretical analysis indicates the hemoglobin fluorescence is mainly due to porphyrin fluorophores in it. In addition, it is found that the peak locations of 476.5 nm laser induced the fluorescence spectra hardly change with the sample concentration from 1% to 7%. The research indicated that the effects of laser are markedly different from that of normal light in the characteristics of low lever light-biotissue interaction.