在经典Gerchberg-Saxton(GS)算法的基础上，提出一种改进的快速三维GS 算法获取目标场的全息图(CGH)并用于全息光镊系统中，从理论和实验上充分证明了该算法的快速性和有效性。实验搭建了一套基于纯相位液晶空间光调制器(SLM)的全息光镊系统，实现了对酵母菌细胞和二氧化硅小球等微粒的多光阱、多平面三维稳定捕获和动态操纵。实验上还产生了具有强度梯度的线状光阱和光学涡旋光阱，实现了对微粒的运输和旋转操纵。这种可以对微粒实现多光阱、多平面动态三维操纵的全息光镊系统为生物、胶体物理等研究提供了一种新的微操纵工具。

An improved and rapid three dimensional Gerchberg-Saxton (GS) algorithm based on the classic GS algorithm for computer generated holograms is proposed and applied to holographic optical tweezers. Theoretical simulations and experimental results have demonstrated the rapidity and efficiency of the proposed algorithm. A robust holographic optical tweezers setup based on phase-only liquid-crystal spatial light modulator(SLM) is built, and stable trapping and dynamic manipulation of yeast cells and silica beads with large array traps in three dimensions are demonstrated. Two special traps, i.e., line trap with intensity gradient distribution and optical vortex trap, are generated to transport and rotate micro-particles respectively. The system is verified to be robust on particles manipulations, which provides a new and powerful tool for researches on biology, colloid physics and so on.