上海航天科工电器研究院有限公司,上海 200331
提出一种基于探针加载的互补开口谐振环(CSRR)的复合左右手传输线(CRLH TL)结构。利用CSRR+CRLH 结构的谐振特性,并通过延长CRLH 耦合缝隙的长度以及增加CSRR 中短路探针的数量,在引入传输零点的同时缩小了滤波器的尺寸。经过仿真优化,实现了滤波器宽频带、高选择性和小型化设计。加工了基于该结构的带通滤波器样机, 样机整体尺寸为30 mm×15 mm×1.35 mm。测试结果表明, 滤波器的中心频率及插入损耗分别为6.6 GHz 和0.65 dB,3 dB 带宽为9.3 GHz,在无线通信、导航等微波系统中具有良好的应用前景。
带通滤波器 复合左右手传输线 互补开口环 短路探针 耦合缝隙 band-pass filter Composite Right/Left-Handed Transmission Lines Complementary Split-Ring Resonator short-circuit probes coupling gaps 太赫兹科学与电子信息学报
2023, 21(6): 814
1 上海理工大学科技发展研究院 技术转移中心,上海 200093
2 上海理工大学 材料与化学学院,上海 200093
设计合成了一种Eu3+离子功能化的聚合物基稀土杂化探针。利用苯甲酰三氟丙酮(BFA)与镧系Eu3+进行配位反应得到配合物Eu(BFA)3后再进一步与MMA单体进行聚合制备得到聚合物杂化探针分子Eu?(BFA)3@PMMA,对Eu(BFA)3@PMMA的结构和荧光性能进行了详细的探究,并且将其用于肿瘤标志物唾液酸(SA)的传感检测应用。研究结果表明,SA能对Eu(BFA)3@PMMA的荧光产生明显的猝灭效果。此外,在最佳激发波长为325 nm时进行荧光性能对比实验发现Eu(BFA)3@PMMA对SA具有较强的选择性和抗干扰能力,且检出限较低。
镧系配合物 杂化探针 唾液酸 荧光传感 lanthanide complexes hybrid probes sialic acid fluorescence sensing
1 南京工业大学 柔性电子(未来技术)学院,江苏 南京 211816
2 南京医科大学 附属肿瘤医院,江苏 南京 210009
活性酶普遍存在于各种生命活动中,一些疾病与活性酶的异常表达息息相关,精确检测酶的表达水平以及原位成像,为相关疾病的诊断与治疗提供了有力的判断依据。至今,大量的检测技术已经开发出来,其中以分子荧光探针为代表的光学技术具有非侵袭性以及灵敏度高、检测限低、响应时间快和生物相容性好等优势,在检测活性酶上备受青睐。然而,在使用分子荧光探针检测时,由于小分子容易在酶活性位点发生扩散,无法定位,导致探针时空分辨率较差。因此,为提高成像检测的时空分辨率、降低背景干扰和假阳性,原位成像的设计理念随之提出,已成为生物光学成像的研究热点之一。目前,研究者已报道多种分子荧光探针用于酶的原位成像的设计并取得显著效果。本文将深入介绍用于活性酶检测的分子荧光探针的设计策略及其在原位成像中的研究进展,希望为该领域的研究者们提供一些启发。
原位成像 分子荧光探针 活性酶 研究进展 in situ imaging small-molecule fluorescent probes enzyme recent progress
1 南通市口腔医院,江苏 南通 226000
2 南通市中西医结合医院,江苏 南通 226000
3 北京大学长三角光电科学研究院,江苏 南通 226000
4 人工微结构和介观物理国家重点实验室,北京大学物理学院,北京 100871
与可见光区(400~700 nm)和近红外一区(NIR-I,700~900 nm)荧光成像(FL)相比,近红外二区(NIR-II,1000~1700 nm)荧光成像具有更深的穿透深度、更高的信噪比。开发亮度高、吸收/发射波长长、生物相容性好的NIR-II荧光探针一直是NIR-II荧光成像领域的一个重要研究方向。有机NIR-II荧光探针以其优异的生物相容性和良好的药代动力学特性而备受关注。本文从红移吸收/发射波长、提高荧光量子产率/摩尔吸光系数、改善生物相容性等角度系统总结了近年来花菁类染料、D-A-D小分子、聚合物点等有机NIR-II荧光探针的研究进展,重点介绍了具有代表性的荧光探针在活体NIR-II荧光成像中的应用,最后讨论了有机NIR-II荧光探针迈向临床应用面临的潜在挑战。
医用光学 近红外二区 荧光探针 花菁类染料 有机小分子 聚集诱导发光 共轭聚合物 中国激光
2023, 50(21): 2107101
1 西湖大学工学院浙江省3D微纳加工和表征研究重点实验室,浙江 杭州 310030
2 浙江西湖高等研究院前沿技术研究所,浙江 杭州 310024
3 浙江大学脑科学与脑医学学院,浙江 杭州 310058
4 浙江大学脑与脑机融合前沿科学中心,浙江 杭州 310058
5 浙江大学光电科学与工程学院,浙江 杭州 310027
6 浙江大学信息与电子工程学院,浙江 杭州 310027
作为光遗传学的重要工具,纳米光遗传探针用于实现对生物体神经元的光刺激,能够辅助神经科学家更具特异性地探索大脑的工作机制,有望用于神经疾病的发病机理分析和治疗。研究人员针对光遗传学刺激的刺激强度、刺激范围、刺激模式、时空分辨率等要求,开发了具有不同光学功能的探针,也针对丰富探针功能如原位电生理记录、化学或生物分子递送等要求,开发了多功能的神经探针。为克服传统光电子器件刚性不可弯折、易对生物体造成损伤等弊端,柔性光学神经探针应运而生。这一类探针在植入时对生物体的损伤小,在植入后能够维持稳定的出光强度,其使用寿命得到保证。本文围绕不同类型、不同功能的光遗传探针以及光遗传探针中的柔性技术进行综述和展望。
光学神经探针 光遗传学 柔性 波导集成型探针 深脑部刺激 生物兼容材料 激光与光电子学进展
2023, 60(13): 1316001
吉林大学电子科学与工程学院集成光电子学国家重点实验室,吉林 长春 130012
脂滴是一种重要的细胞器,与细胞中多种生理活动密切相关。为了观察脂滴并研究其多种多样的功能,共聚焦荧光成像技术是最有力的工具之一。然而,细胞脂滴荧光成像所需的具有高荧光亮度和高标记选择性的脂滴荧光探针却十分有限,这严重限制了脂滴的深入研究。研制了一种具有荧光开关特性的喹啉衍生物脂滴荧光探针(Lipi-QL)。该探针因其敏感的极性淬灭荧光特性,展现出了很高的脂滴标记选择性。Lipi-QL能靶向脂滴是因为其脂溶性,进入脂滴后,荧光增强是脂滴内的较低极性引起的。给受体型分子结构还赋予了该探针高的荧光亮度以及大的斯托克斯位移。将该探针用于细胞脂滴共聚焦荧光成像,在不同浓度下都实现了显著优于脂滴商用探针BODIPY 493/503的标记选择性。此外,使用该荧光探针实现了固定细胞的三维共聚焦成像和活细胞的四色共聚焦成像。该荧光探针的研制一方面为脂滴生理功能的研究提供了强有力的工具,另一方面也为新型高标记选择性荧光探针的设计提供了新的思路。
医用光学 荧光开关特性 荧光成像 荧光探针 脂滴
Author Affiliations
Abstract
1 Jerusalem College of Technology, Lev Academic Center, Faculty of Engineering, Department of Applied Physics/Electro-Optics Engineering, Advanced Laboratory of Electro-Optics, Jerusalem, Israel
2 Bar-Ilan University, Faculty of Engineering, Ramat Gan, Israel
3 Nanotechnology Center for Research and Education, Lev Academic Center, Jerusalem, Israel
4 Bar-Ilan University, The Nanotechnology Center, Ramat Gan, Israel
5 Politehnica University of Bucharest, Center for Microscopy-Microanalysis and Information Processing, Bucharest, Romania
A thorough understanding of biological species and emerging nanomaterials requires, among other efforts, their in-depth characterization through optical techniques capable of nanoresolution. Nanoscopy techniques based on tip-enhanced optical effects have gained tremendous interest over the past years, given their potential to obtain optical information with resolutions limited only by the size of a sharp probe interacting with focused light, irrespective of the illumination wavelength. Although their popularity and number of applications is rising, tip-enhanced nanoscopy (TEN) techniques still largely rely on probes that are not specifically developed for such applications, but for atomic force microscopy. This limits their potential in many regards, e.g., in terms of signal-to-noise ratio, attainable image quality, or extent of applications. We take the first steps toward next-generation TEN by demonstrating the fabrication and modeling of specialized TEN probes with known optical properties. The proposed framework is highly flexible and can be easily adjusted to be used with diverse TEN techniques, building on various concepts and phenomena, significantly augmenting their function. Probes with known optical properties could potentially enable faster and more accurate imaging via different routes, such as direct signal enhancement or facile and ultrafast optical signal modulation. We consider that the reported development can pave the way for a vast number of novel TEN imaging protocols and applications, given the many advantages that it offers.
tip-enhanced nanoscopy scanning probes COMSOL simulations nano-objects optical modeling Advanced Photonics Nexus
2023, 2(2): 026002
Author Affiliations
Abstract
1 Institute of Photonic Chips, University of Shanghai for Science and Technology, Shanghai 200093, P. R. China
2 Centre for Artificial-Intelligence Nanophotonics, School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, P. R. China
The MINimal emission FLUXes (MINFLUX) technique in optical microscopy, widely recognized as the next innovative fluorescence microscopy method, claims a spatial resolution of 1–3nm in both dead and living cells. To make use of the full resolution of the MINFLUX microscope, it is important to select appropriate fluorescence probes and labeling strategies, especially in living-cell imaging. This paper mainly focuses on recent applications and developments of fluorescence probes and the relevant labeling strategy for MINFLUX microscopy. Moreover, we discuss the deficiencies that need to be addressed in the future and a plan for the possible progression of MINFLUX to help investigators who have been involved in or are just starting in the field of super-resolution imaging microscopy with theoretical support.The MINimal emission FLUXes (MINFLUX) technique in optical microscopy, widely recognized as the next innovative fluorescence microscopy method, claims a spatial resolution of 1–3nm in both dead and living cells. To make use of the full resolution of the MINFLUX microscope, it is important to select appropriate fluorescence probes and labeling strategies, especially in living-cell imaging. This paper mainly focuses on recent applications and developments of fluorescence probes and the relevant labeling strategy for MINFLUX microscopy. Moreover, we discuss the deficiencies that need to be addressed in the future and a plan for the possible progression of MINFLUX to help investigators who have been involved in or are just starting in the field of super-resolution imaging microscopy with theoretical support.
Fluorescence probes MINFLUX nanoscopy photoblinking super-resolution imaging labeling strategy Journal of Innovative Optical Health Sciences
2023, 16(1): 2230011