在生物医学领域,为了降低成本,降低对高端器件的依赖,以及实现对无标记样本的光谱和结构等多维度图像分析的目的,基于窄带LED光源技术,自主研制了一套多通道LED照明的多光谱显微成像系统。本系统在420~680nm范围内系统的光谱分辨率约为20nm,空间分辨率优于2μm,在放大倍数为13倍时成像范围为520μm × 416μm。为了验证系统在临床病理分析中的可行性,采集了小鼠皮肤鳞状细胞原位癌病变病理切片和正常皮肤组织病理切片的多光谱图像。从多光谱图像中可以清楚地观察结构,由图像序列中提取的光谱信息表明,癌变细胞核的反射率在可见光波段内与正常细胞核有明显差异,能有效区分两种细胞。这些实验结果表明,基于LED照明的多光谱显微成像系统有望替代传统昂贵、复杂的多光谱成像系统,并可在病理分析中发挥重要作用。

In the biomedical field, to reduce the cost and dependence on advanced devices and to realize multi-dimensional image analysis for unlabeled samples, including spectra and structures, a multispectral microimaging system with multi-channel LEDs was independently developed based on narrow-band LED light source technology. The spectral resolution of the system was 20nm over 420--680nm, with spatial resolution better than 2μm and an imaging range of 520μm × 416μm under 13× magnification. To verify feasibility of the system for clinicopathological analysis, multispectral images of mouse skin squamous cell carcinomas were collected, for both in situ and normal skin tissues. Excitingly, cell structure was clearly observed using the multispectral image system. Spectral information extracted from the image sequence shows that reflectance of the cancerous nucleus has significant difference with that of the normal nucleus in the visible band, allowing the two types of cells to be effectively distinguished. These results indicate that LED illumination-based multispectral imaging systems are promising alternatives to replace traditional, expensive, and complex multispectral imaging systems and play an important role in pathological analysis.