To improve the optical performance of an antireflection (AR) coating on a micro-spherical substrate, the ray angle of the incidence distribution and the thickness profile are taken into consideration during the optical coating design. For a convex spherical substrate with a radius of curvature of 10 mm and a clear aperture of 10 mm, three strategies are used for the optimization of the spectral performance of a broadband AR coating in the spectral region from 480 to 720 nm. By comparing the calculated residual reflectance and spectral uniformity, the developed method demonstrates its superiority in spectral performance optimization of an AR coating on a micro-spherical substrate.