为了探究光清透剂在减少皮肤组织散射，提高到达靶组织激光能量方面的作用，利用皮肤仿体定量研究了可见-近红外波长下甘油的光清透效果。搭建了葡萄酒色斑激光治疗的体外模拟实验系统，研究光清透效果与多脉冲Nd∶YAG激光作用下血液凝结特性之间的定量关系。结果表明，0.5 mL无水甘油涂抹在仿体表面10 min后，在波长1064 nm处仿体的漫反射率降低36.69%，透射率增加38.73%。将无水甘油涂抹在仿体表面4 min后，仿体下毛细血管中血液凝结所需激光脉冲数目减少了25%。作用10 min后，所需激光脉冲数目并未进一步减少，但血液凝结面积与4 min时相比增加了34.1%。这说明甘油能有效提高病变血管对激光能量的吸收，改善葡萄酒色斑的疗效。

In order to study the optical clearing effect on reducing the skin scattering and increasing the laser energy reaching the target vessel, a quantitative study on the optical clearing effect of glycerol on the skin tissue phantom was conducted in the visible to infrared wavelength band. An in vitro experimental system for laser treatment of port wine stain was set up to obtain quantitative relationship between optical clearing effect and the blood coagulation properties under the irradiation of multi-pulse Nd∶YAG laser. The results show that the diffuse reflectance of the skin tissue phantom decreases by 36.69% and the transmittance increases by 38.73% at 1064 nm after 0.5 mL anhydrous glycerol is applied on the skin phantom surface for 10 min. After 0.5 mL anhydrous glycerol is applied on the skin tissue phantom surface for 4 min, the number of laser pulses required for blood coagulation decreases by 25%. After application for 10 min, the number of laser pulses required for blood coagulation does not decrease further, but the blood coagulation area increases by 34.1% compared with that after 4 min. The results indicate that glycerol is effective to improve the laser treatment of port wine stain by increasing the laser energy reaching the target vessel.