光学学报, 2017, 37 (3): 0318002, 网络出版: 2017-03-08
结构导向的可逆光激活绿色荧光蛋白探针的研制 下载: 942次
Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe
显微 光学显微术 可逆光激活 荧光蛋白 超分辨成像 荧光探针 绿色荧光蛋白 microscopy optical microscopy reversibly photoswitchable fluorescent protein super-resolution imaging fluorescent probe green fluorescent protein
摘要
近几年, 可逆光激活荧光蛋白的研制越来越受到人们的重视, 这类荧光蛋白极大地促进了活细胞超高分辨显微成像技术的发展及应用。可逆光激活荧光蛋白可被不同波长的光多次可逆地进行调制, 因而被广泛地应用于高密度数据的光存储、光致变色荧光共振能量转移的测量以及基于可逆饱和线性荧光跃迁原理的超高分辨率显微成像中。从研制这类荧光蛋白所涉及的关键氨基酸位点出发, 本文综述了近几年可逆光激活绿色荧光蛋白的研制进展, 并简要地讨论荧光蛋白结构与光学特性的关系, 从而为后续结构导向的可逆光激活荧光蛋白的研制提供参考。
Abstract
In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of live-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by different wavelength lights, thus it can be widely applied in many fields such as high density data optical storage, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on reversible saturable linear fluorescence transitions (RESOLFT) principle. The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs.
王盛, 陈轩泽, 常蕾, 薛瑞莹, 孙育杰. 结构导向的可逆光激活绿色荧光蛋白探针的研制[J]. 光学学报, 2017, 37(3): 0318002. Wang Sheng, Chen Xuanze, Chang Lei, Xue Ruiying, Sun Yujie. Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe[J]. Acta Optica Sinica, 2017, 37(3): 0318002.