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基于太赫兹光谱技术的生物膜界面水研究

Terahertz Spectroscopy of the Interfacial Water in Phospholipid Membranes

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摘要

界面水对生物膜的功能发挥起着至关重要的作用,但由于水对太赫兹波有极强的吸收,限制了液体样品的透射谱研究。将磷脂包覆水球的制备方法引入太赫兹时域光谱技术探测界面水的实验体系中,不仅大大减少了所需被测样品的用量,而且可调节水对太赫兹的吸收程度。基于此方法,在获得多种磷脂乳液的太赫兹介电谱的基础上,揭示了磷脂极性头部的化学基团差异会明显影响其界面的水合状态及界面水分子的动力学行为。

Abstract

Interfacial water plays an important role in the function of biological membranes. However, due to the significant absorption of water on the terahertz wave, it is very difficult to obtain the terahertz transmission spectra of the membrane interfaces. Herein, we develop a system to study the membrane interfacial water by combining the preparation of water droplets in lipid and terahertz time domain spectroscopy. It only needs a few biological samples but has high sensitivity of water state in this method. Based on this system, we have successfully measured the terahertz dielectric spectra of kinds of lipid emulsions. The results revealed that the hydration state and water dynamics upon the membrane interface are dependent on the lipid headgroup components.

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中图分类号:O433.4

DOI:10.3788/lop54.043001

所属栏目:光谱学

基金项目:国家自然科学基金(11474298)、中国科学院前沿科学重点研究项目(QYZDJ-SSW-SLH019)

收稿日期:2016-10-08

修改稿日期:2016-12-26

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潘亚涛:上海大学理学院, 上海 200444中国科学院上海应用物理研究所微观界面物理与探测重点实验室, 上海 201800
吕军鸿:中国科学院上海应用物理研究所微观界面物理与探测重点实验室, 上海 201800

联系人作者:潘亚涛(1229241441@qq.com)

备注:潘亚涛(1990-),男,硕士研究生,主要从事生物膜界面水方面的研究。

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引用该论文

Pan Yatao,Lü Junhong. Terahertz Spectroscopy of the Interfacial Water in Phospholipid Membranes[J]. Laser & Optoelectronics Progress, 2017, 54(4): 043001

潘亚涛,吕军鸿. 基于太赫兹光谱技术的生物膜界面水研究[J]. 激光与光电子学进展, 2017, 54(4): 043001

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