激光与光电子学进展, 2017, 54 (4): 040901, 网络出版: 2017-04-19
基于两步相移的非相干数字全息显微技术 下载: 552次
Two-Step Phase-Shifting Based Incoherent Digital Holographic Microscopy
全息 非相干全息 小波变换 两步相移 荧光全息显微 holography incoherent holography wavelet transform two-step phase-shifting fluorescent holographic microscopy
摘要
基于小波变换抑制偏置量,并利用初始图像消除噪声,进行非相干全息两步相移重现。给出了全息图重现及抑制偏置量计算的具体表达式。实验搭建了基于空间光调制器的非相干数字全息显微系统,给出基于三步相移技术和两步相移技术的数字全息图和重现结果,并对比了两种不同方法的两步相移重现结果。对荧光显微颗粒拍摄了两张全息图,并在不同平面实现了数字重聚焦。两步相移相比三步相移成像速度大大提高,为活体细胞动态三维成像提供可能。实验结果表明利用小波变换及初始图像能够更有效地抑制偏置量并消除噪声,在不增加相移次数的前提下,提高两步相移的再现像质量。
Abstract
Two-step phase-shifting based on wavelet transform to suppress bias term is reported, and the initial image is used to eliminate background noise. Specific forms of reconstructing hologram and suppressing bias term are given. An incoherent digital holographic microscopy system based on spatial light modulator is built and the holograms and reconstruction results of both three-step phase-shifting and two-step phase-shifting are given. Reconstruction results of the two methods are compared. Experiment captures two holograms of the microscopic fluorescent beads which realize digital reconstruction in different planes using the method presented in this paper. Two-step phase-shifting significantly enhances imaging speed compared to three-step phase-shifting, and provides the possibility to image 3D living cells dynamically. The result shows that wavelet transform can suppress bias term more effectively. We improve the quality of reconstructed image by two-step phase-shifting without increasing the phase-shifting step.
李滢滢, 秦琬, 高志, 彭翔. 基于两步相移的非相干数字全息显微技术[J]. 激光与光电子学进展, 2017, 54(4): 040901. Li Yingying, Qin Wan, Gao Zhi, Peng Xiang. Two-Step Phase-Shifting Based Incoherent Digital Holographic Microscopy[J]. Laser & Optoelectronics Progress, 2017, 54(4): 040901.