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基于两步相移的非相干数字全息显微技术

Two-Step Phase-Shifting Based Incoherent Digital Holographic Microscopy

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摘要

基于小波变换抑制偏置量,并利用初始图像消除噪声,进行非相干全息两步相移重现。给出了全息图重现及抑制偏置量计算的具体表达式。实验搭建了基于空间光调制器的非相干数字全息显微系统,给出基于三步相移技术和两步相移技术的数字全息图和重现结果,并对比了两种不同方法的两步相移重现结果。对荧光显微颗粒拍摄了两张全息图,并在不同平面实现了数字重聚焦。两步相移相比三步相移成像速度大大提高,为活体细胞动态三维成像提供可能。实验结果表明利用小波变换及初始图像能够更有效地抑制偏置量并消除噪声,在不增加相移次数的前提下,提高两步相移的再现像质量。

Abstract

Two-step phase-shifting based on wavelet transform to suppress bias term is reported, and the initial image is used to eliminate background noise. Specific forms of reconstructing hologram and suppressing bias term are given. An incoherent digital holographic microscopy system based on spatial light modulator is built and the holograms and reconstruction results of both three-step phase-shifting and two-step phase-shifting are given. Reconstruction results of the two methods are compared. Experiment captures two holograms of the microscopic fluorescent beads which realize digital reconstruction in different planes using the method presented in this paper. Two-step phase-shifting significantly enhances imaging speed compared to three-step phase-shifting, and provides the possibility to image 3D living cells dynamically. The result shows that wavelet transform can suppress bias term more effectively. We improve the quality of reconstructed image by two-step phase-shifting without increasing the phase-shifting step.

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中图分类号:O438

DOI:10.3788/lop54.040901

所属栏目:全息

基金项目:国家自然科学基金(61377017,61201355)

收稿日期:2016-11-30

修改稿日期:2016-12-21

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作者单位    点击查看

李滢滢:深圳大学光电工程学院教育部/广东省光电子器件与系统重点实验室, 广东 深圳 518060深圳职业技术学院电子与通信工程学院, 广东 深圳 518055
秦琬:美国克莱姆森大学生物工程系, 南卡罗来纳州 克莱姆森 29634, 美国
高志:美国克莱姆森大学生物工程系, 南卡罗来纳州 克莱姆森 29634, 美国
彭翔:深圳大学光电工程学院教育部/广东省光电子器件与系统重点实验室, 广东 深圳 518060

联系人作者:李滢滢(coolwind85@szpt.edu.cn)

备注:李滢滢(1985-),女,博士研究生,讲师,主要从事全息显微镜、三维数字成像方面的研究。

【1】Gabor D. A new microscopic principle[J]. Nature, 1948, 161(4098): 777.

【2】Garcia-Sucerquia J, Xu W, Jericho S K, et al. Digital in-line holographic microscopy[J]. Applied Optics, 2006, 45(5): 836-850.

【3】Rosen J, Brooker G. Digital spatially incoherent Fresnel holography[J]. Optics Letters, 2007, 32(8): 912-914.

【4】Rosen J, Brooker G. Fluorescence incoherent color holography[J]. Optics Express, 2007, 15(5): 2244-2250.

【5】Rosen J, Brooker G. Non-scanning motionless fluorescence three-dimensional holographic microscopy[J]. Nature Photonics, 2008, 2(3): 190-195.

【6】Rosen J, Katz B, Brooker G. Fresnel incoherent correlation hologram - a review[J]. Chinese Optics Letters, 2009, 7(12): 1134-1141.

【7】Katz B, Rosen J, Kelner R, et al. Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH) using dual diffractive lenses on a spatial light modulator (SLM)[J]. Optics Express, 2012, 20(8): 9109-9121.

【8】Brooker G, Siegel N, Wang V, et al. Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy[J]. Optics Express, 2011, 19(6): 5047-5062.

【9】Katz B, Rosen J. Could SAFE concept be applied for designing a new synthetic aperture telescope[J]. Optics Express, 2011, 19(6): 4924-4936.

【10】Kashter Y, Rosen J. Enhanced-resolution using modified configuration of Fresnel incoherent holographic recorder with synthetic aperture[J]. Optics Express, 2014, 22(17): 20551-20565.

【11】Katz B, Rosen J, Kelner R, et al. Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH) using dual diffractive lenses on a spatial light modulator (SLM)[J]. Optics Express, 2012, 20(8): 9109-9121.

【12】Yamaguchi I, Zhang T. Phase-shifting digital holography[J]. Optics Letters, 1997, 22(16): 1268-1270.

【13】Qin W, Yang X Q, Li Y Y, et al. Two-step phase-shifting fluorescence incoherent holographic microscopy[J]. Journal of Biomedical Optics, 2014, 19(6): 0605003.

【14】Wan Q, Yang X Q, Wang Y R, et al. Fast fluorescence holographic microscopy[C]. SPIE, 2014, 8949: 89491W.

【15】Meng X F, Cai L Z, Xu X F, et al. Wavefront reconstruction by two-step generalized phase-shifting interferometry[J]. Optics Communications, 2008, 281(23): 5701-5705.

【16】Meng X F, Cai L Z, Xu X F, et al. Two-step phase-shifting interferometry and its application in image encryption[J]. Optics Letters, 2006, 31(10): 1414-1416.

【17】Wan Yuhong, Man Tianlong, Tao Shiquan. Imaging characteristics and research progress of incoherent holography[J]. Chinese J Lasers, 2014, 41(2): 0209004.
万玉红, 满天龙, 陶世荃. 非相干全息术成像特性及研究进展[J]. 中国激光, 2014, 41(2): 0209004.

【18】Liu Yingchen, Fan Jinping, Zeng Fanchuang, et al. Recording, reconstruction and realization of white-light Fresnel incoherent digital holography[J]. Chinese J Lasers, 2013, 40(10): 1009002.
刘英臣, 范金坪, 曾凡创, 等. 白光菲涅耳非相干数字全息的记录、再现及实现[J]. 中国激光, 2013, 40(10): 1009002.

【19】Shi Xia, Zhu Wufeng, Yuan Bin, et al. Experimental study of the incoherent digital holography[J]. Chinese J Lasers, 2015, 42(12): 1209003.
石 侠, 朱五凤, 袁 斌, 等. 非相干光照明数字全息实验研究[J]. 中国激光, 2015, 42(12): 1209003.

【20】Huang Jingjing, Chen Wenjing, Su Xianyu, et al. Application of wavelet transform in modulation measurement profilometry[J]. Acta Optica Sinica, 2016, 36(7): 0707001.
黄静静, 陈文静, 苏显渝, 等. 小波变换在调制度测量轮廓术中的应用[J]. 光学学报, 2016, 36(7): 0707001.

【21】Li Qingzhong, Liu Qing. Adaptive enhancement algorithm for low illumination images based on wavelet transform[J]. Chinese J Lasers, 2015, 42(2): 0209001.
李庆忠, 刘 清. 基于小波变换的低照度图像自适应增强算法[J]. 中国激光, 2015, 42(2): 0209001.

【22】Wang Cunshuai, Zhang Yinke, Hao Jinbo, et al. Improving reconstruction image quality of digital holography using median filter and intensity subtraction[J]. Laser & Optoelectronics Progress, 2011, 48(12): 120901.
王存帅, 张引科, 郝劲波, 等. 用中值滤波和光强相减法改善数字全息再现像质量[J]. 激光与光电子学进展, 2011, 48(12): 120901.

【23】Wang Liang, Feng Shaotong, Nie Shouping, et al. Improving the reconstructed image quality of the digital holography through multiscale transform[J]. Journal of Optoelectronics·Laser, 2007, 18(5): 625-628.
王 亮, 冯少彤, 聂守平, 等. 利用多尺度变换提高三维物体数字全息再现像质量[J]. 光电子·激光, 2007, 18(5): 625-628.

【24】Zeng Taiying, Shao Xue, Wang Zuhui, et al. No-reference blur image quality assessment based on local discrete wavelet transform[J]. Journal of Optoelectronics·Laser, 2016, 12(7): 754-760.
曾台英, 邵 雪, 汪祖辉, 等. 基于局部离散小波变换的无参考模糊图像质量评价方法[J]. 光电子·激光, 2016, 12(7): 754-760.

【25】Xu Dongying, Li Sikun, Wang Xiangzhao, et al. Wavelet ridge extraction method employing a cost function in two-dimensional wavelet transform profilometry[J]. Acta Optica Sinica, 2016, 36(4): 0412006.
徐东瀛, 李思坤, 王向朝, 等. 基于评价函数的二维小波变换轮廓术小波脊提取方法[J]. 光学学报, 2016, 36(4): 0412006.

引用该论文

Li Yingying,Qin Wan,Gao Zhi,Peng Xiang. Two-Step Phase-Shifting Based Incoherent Digital Holographic Microscopy[J]. Laser & Optoelectronics Progress, 2017, 54(4): 040901

李滢滢,秦琬,高志,彭翔. 基于两步相移的非相干数字全息显微技术[J]. 激光与光电子学进展, 2017, 54(4): 040901

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