Chinese Optics Letters, 2017, 15 (9): 090003, Published Online: Jul. 19, 2018  

Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy Download: 1091次

Author Affiliations
1 Britton Chance Center for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology, Wuhan 430074, China
2 MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Abstract
To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67, we demonstrate a custom-built second harmonic generation (SHG) microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG image. Further, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.

Xiuli Liu, Daozhu Hua, Ling Fu, Shaoqun Zeng. Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy[J]. Chinese Optics Letters, 2017, 15(9): 090003.

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