设计了基于数字微镜器件(DMD)的荧光显微镜探测系统。该系统不仅能对样品实现荧光成像, 而且能探测不同区域样品激发荧光信号能量的实时变化。实验采用DMD快速加载多通道不同帧率的视频信息, 实现了对激发光束的分割与频率调制。采用光电倍增管采集样品的荧光信息, 荧光信息经模/数转换处理后再进行傅里叶变换、滤波和解调操作, 得到了不同信道区域荧光信号强度随时间变化的曲线。实验结果表明, 将DMD加入到荧光显微镜系统中可以加快集成多通道, 且计算机可调控DMD通道, 能有效提高荧光显微镜的探测效率。

A fluorescence microscope detection system based on digital micromirror device (DMD) is designed. The system not only can realize the fluorescence imaging of the sample, but also can detect the real-time change of the fluorescence signal energy in different regions of the sample. The video information with different frame rates in multi-channel is fast loaded by DMD in the experiment to realize the segmentation of the excitation beam and frequency modulation. The fluorescence information of the sample is collected by photomultiplier tube (PMT) and then is processed by means of analog-to-digital conversion, Fourier transform, filtering and demodulation, and the relationship curve between intensity of fluorescence signal in different channel regions and time. The experimental results show that adding DMD to fluorescence microscope system can accelerate the integration of multi-channel, the DMD channel can be controlled by computer, and the detection efficiency of the fluorescence microscope can be improved effectively.