CRISPR/Cas9是一种多功能的基因工程技术, 除了基因编辑, 在基因表达的调控, 活细胞DNA/RNA成像等方面也受到了广泛的关注。然而, CRISPR/Cas9系统源自原核生物, 要应用到其他生物体内, 还需要有运载体的协助。本文结合CRISPR/Cas9技术在多方面的应用, 对其运载体进行了综述, 为相关领域的工作提供参考。

CRISPR/Cas9 is a versatile gene engineering technology that has been received extensive attention in gene editing, regulation of gene expression and live cell DNA/RNA imaging as well. However, the CRISPR/Cas9 system is derived from prokaryotes, which requires delivery vehicles for application in other organisms. Combined with a variety of applications of CRISPR/Cas9 technology, this paper reviews its delivery vehicles to providing a reference to the work of relevant fields.