中国激光, 2018, 45 (2): 0207030, 网络出版: 2018-02-28
基于黑磷荧光淬灭特性的肿瘤细胞探测 下载: 1095次
Detection of Cancer Cells Based on Fluorescence Quenching Property of Black Phosphorus
医用光学 乳腺癌细胞检测 荧光淬灭 核酸适体 medical optics breast cancer cell detection fluorescence quenching DNA aptamer
摘要
黑磷(BP)纳米片可淬灭荧光染料的荧光,基于这一特性,合成了可特异性识别人乳腺癌细胞MCF7的脱氧核糖核酸(DNA)适体,并用荧光染料5-羧基荧光素(FAM)将其标记。当BP纳米片与该适体混合时,其荧光被淬灭。而当MCF7细胞存在时,DNA适体由于可以识别该细胞并与之结合,会从BP纳米片上脱离,从而实现荧光恢复。恢复的荧光强度与细胞数量呈线性关系。实验结果表明,利用BP纳米片检测乳腺癌细胞具有高效、特异、灵敏的优势,这一方法可以推广至其他肿瘤细胞的检测。
Abstract
Black phosphorus (BP) nanosheets can quench the fluorescence of a fluorescent dye, and based on this property, the deoxyribonucleic acid(DNA) aptamer which can specifically recognize the human breast cancer cells MCF7 are synthesized and labeled with 5-carboxyfluorescein (FAM). When the DNA aptamer is mixed with BP nanosheets, its fluorescence is quenched. However, in the presence of MCF7 cells, the DNA aptamer can recognize the MCF7 cells and interact with these cells, and the disassociation of DNA aptamer from BP nanosheets occurs and then the restoration of fluorescence is realized. The fluorescence intensity shows the linear relationship with the number of cancer cells. The experimental results indicate that it is highly efficient, specific and sensitive to use BP nanosheets for detecting breast cancer cells and this method can be applied in the detection of other kinds of cancer cells.
闫武娟, 王秀翃, 姚倩, 乔鹏飞, Lang Marion C. 基于黑磷荧光淬灭特性的肿瘤细胞探测[J]. 中国激光, 2018, 45(2): 0207030. Yan Wujuan, Wang Xiuhong, Yao Qian, Qiao Pengfei, Lang Marion C. Detection of Cancer Cells Based on Fluorescence Quenching Property of Black Phosphorus[J]. Chinese Journal of Lasers, 2018, 45(2): 0207030.