随着荧光探针的出现,超分辨成像技术发展迅速,多种不同的超分辨显微技术产生并得到广泛应用。超分辨成像技术的发展为生命科学领域的研究提供了极大便利,也越来越受到研究者的重视。由于传统超分辨显微成像技术在成像速度、观察视野范围、系统成本等方面仍存在一定的限制,新型超分辨显微成像技术的发展让研究人员再次看到超分辨成像领域的曙光。介绍了近年来出现的几种新型超分辨成像技术,包括膨胀样品超分辨技术、表面增强超分辨技术和荧光偏振超分辨技术,旨在总结这些新型超分辨技术的发展,为生命科学领域提供新的技术参考。

With the advent of fluorescent probes, the technology of super-resolution imaging has developed rapidly, and a variety of different super-resolution microscopy technologies have been developed and widely used. It provides great convenience for the field of life science research and receives more and more attention from researchers. Because the traditional super-resolution microscopic imaging technologies still have some restrictions in imaging speed, observation field of view, system costs and so on, the development of new super-resolution microscopy technology expands our vision of the field of super-resolution image. Several new super-resolution imaging technologies including expansion sample super-resolution, surface enhanced super-resolution and fluorescence polarization super-resolution are reviewed with the purpose of making a summary of the development of new super-resolution technologies and providing a new technical reference for the application of the field of life science.