为探索一种能够全面快速的鉴定瘤背石磺质量的方法, 采用红外光谱法(IR)建立六个不同产地瘤背石磺肌肉组织无水乙醇、 氯仿、 乙酸乙酯三种提取物的红外指纹图谱, 使用Nicolet 5700红外光谱仪, 结合OPUS分析软件, 发现三种提取物的红外指纹图谱可以用于不同产区瘤背石磺的质量评价与产地溯源, 其相似度值的大小可作为石磺产地的评价指标, 三种提取物的部分特征峰对比, 乙酸乙酯提取物的红外指纹图谱能体现出更全面的化学成分信息。 三种提取物对瘤背石磺的鉴定体现程度及角度有所不同, 双指标法比较显示, 氯仿提取物能够更好体现出不同产地、 不同样品处理的瘤背石磺间的差异: 对于产地相近的SH和ND, WZ和XM的样品共有峰率≥63.4, 变异峰率小于等于38.5; 产地相距较远的SH和YN, YC和XM的样品共有峰率≤40.9, 变异峰率≥50.0; 产地相近但样品前处理方式不同的XM和YN共有峰率为38.1, 而变异峰率高达125.0, 综合分析得出, 利用氯仿提取物的共有峰率和变异峰率结合双指标序列分析法可以快速、 准确鉴定不同产地、 不同处理方式的瘤背石磺。

Aiming to establish a comprehensive and rapid method to discriminate Onchidium struma（O. struma） quality from different regions, this research has established it’s three extract fingerprints in absolute ethyl alcohol, chloroform and ethyl acetate adoptd the infrared spectrometry (IR), and the information of infrared spectra of O. struma samples originated from 6 regions has been collected. Automatic baseline correction and automatic optimized smoothness of Nicolet 5700 infrared spectrometer intelligence system are applied to optimize the IR fingerprint. Combining with the OPUS software. The results showed that, the three extract fingerprints function as a evaluation index to discriminate O. struma from different regions or different quality. The characteristic absorption peaks of three extracts has been contrasted, reflecting that the IR fingerprint spectra of ethyl acetate extract can reflect more abundant chemical composition information. The three extracts have different level and angle for identification of O. struma. Dual-indexes sequential analysis comparison shows that infrared spectrum of chloroform extract can reflect different habitats and different process mode O. struma differences: It has shown ShangHai（SH）and NingDe（ND）, WenZhou（WZ）and XiaMen（XM）were the region closer samples with higher common peak ratio（≥63.4）and lower variant peak ratio（≤38.5）; Sample SH and YueNan（YN）,YanCheng（YC）and XiaMen（XM）from the farther regions, were significant disparity with lower common peak ratio（≤40.9％）and higher variant peak ratio（≥50.0％）; In addition, sample XM and YN from closer region but have different process mode, which resulted in much lower common peak ratio（38.1％）and much higher variant peak ratio（125.0％）, To sum up, the quality control of aquatic products is a major concern for both the health authorities and the public, considering that current commercially available O. struma are acquired in natural way, acquiring area and time leading to the vital difference of their quality. The method of chloroform extracts dual-index sequence analysis could be used exactly and conveniently in qualitative evaluation of different region samples, and clarified the similarity level among different areas, also applied the quality evaluation of O. struma.