建立薄层色谱（TLC）与拉曼光谱联用方法, 并对减肥类保健食品中非法添加的四种化学成分（茶碱、 咖啡因、 苯丙酸诺龙、 螺内酯）进行检测。 采用TLC法将掺杂成分与保健食品基质进行初步分离, 在紫外灯（254 nm）下检视定位, 以780 nm激光为光源, 对TLC上微量掺杂成分的斑点原位, 直接检测浓集点的拉曼光谱。 考察不同配方保健食品基质对掺杂成分拉曼光谱的影响, 并对检测限进行验证, 初步建立了减肥类保健食品中四种掺杂成分的TLC原位拉曼光谱检测方法。 常用保健食品基质对掺杂成分检测结果无干扰, 检测限为2~4 μg, 在九种减肥类保健食品中检测出两种含有咖啡因。 该研究建立了TLC与拉曼光谱联用技术并采用此方法快速鉴别了减肥保健食品中茶碱等四种非法添加化学成分, 本法专属、 灵敏、 快速、 简便, 可为保健食品中非法添加化学成分的快速检测技术研究提供新的参考依据。

To establish a thin layer chromatography (TLC) combined with Raman spectroscopy method and detect four chemical components (theophylline, caffeine, nandrolone phenylpropionate, spironolactone) added illegally in the slimming health food. The doping ingredient and substrate were separated with TLC, viewed and located with UV light (254 nm), the spot of the doping ingredient is concentrated on the TLC plate before detecting the Raman spectroscopy of the concentrated point directly with 780 nm laser source. The effects of different formulations of slimming health food were studied, the limit of detect (LOD) was validated, and the TLC Situ Raman Spectroscopy method was established initially for detecting four chemical components added in the slimming health food. The common substrate in the slimming health food had no interference with the detecting results of doping components. The LOD was 2~4 μg. Two out of nine kinds of health food were detected to be with caffeine. In this study, we established TLC and Raman spectroscopy for the first time which was used for quick identification of four kinds of illegal chemicals added in health food, such as theophylline, etc. The method is precise, sensitive, rapid and simple, which could provide a new reference for the technology research of rapid detection of the ingredients added illegally in health food.