激光与光电子学进展, 2018, 55 (5): 050003, 网络出版: 2018-09-11
线扫描共聚焦成像技术在生物医学成像中的应用 下载: 2356次
Application of Confocal Line Scanning Imaging Technique in Biomedical Imaging
显微 线扫描共聚焦 共聚焦显微镜 眼底成像 生物医学成像 在体成像 microscopy confocal line scanning confocal microscope fundus imaging biomedical imaging in vivo imaging
摘要
线扫描共聚焦成像技术基于共聚焦成像原理,使用线光束一维扫描照明样品以提高成像速率;通过共焦狭缝滤除样品成像光束中的非聚焦层面杂散光,提高成像分辨率和对比度;近年来,该技术因分辨率高、成像快、成像视场大、系统结构简单等优点而在生物医学成像中的应用越来越广泛。介绍了线扫描共聚焦成像技术的基本原理,列举了成像系统的主要参数及其影响因素,并举例说明了其在生物医学成像,尤其是眼底成像和生物组织细胞观察等方面的应用,最后总结了该技术的优缺点及其应用前景。
Abstract
Based on confocal imaging principle, the confocal line scanning imaging technique uses the one-dimensional scanning-line beam to illuminate the sample for increasing imaging speed. A confocal slit is used to filter the stray light from unfocused-level for improving imaging resolution and contrast ratio. Recently, the confocal line scanning imaging technology is becoming more and more popular in biomedical imaging because of its advantages such as high imaging speed, high imaging resolution, simple system structure, and so on. We introduce the basic principle of confocal line scanning imaging, and list main parameters of the imaging system and their influencing factors. Then the application in biomedical imaging especially in fundus imaging and biological tissue cell observation is illustrated. Advantages, disadvantages, and application prospect of the technique are finally summarized.
孔文, 高峰, 樊金宇, 史国华. 线扫描共聚焦成像技术在生物医学成像中的应用[J]. 激光与光电子学进展, 2018, 55(5): 050003. Wen Kong, Feng Gao, Jinyu Fan, Guohua Shi. Application of Confocal Line Scanning Imaging Technique in Biomedical Imaging[J]. Laser & Optoelectronics Progress, 2018, 55(5): 050003.