研究应用光声-光学相干层析成像(PA/OCT)双模式成像方法测量血流绝对速度和角度。在双模式成像方法中,利用光声相关谱方法测量血流速度在垂直于探测激光光束传播方向的速度分量,利用多普勒光学相干层析成像测量血流速度在平行于探测激光光束传播方向的速度分量,最终得到血流绝对速度和角度。在不同倾斜角度条件下,针对绝对速度为1 mm·s ^-1的血液样品,PA/OCT测得流速结果的标准差为0.02 mm·s ^-1,测得的血流角度与实际样品倾斜角度的相关系数为0.997。实验结果表明,PA/OCT可用于测量血流绝对速度和角度。

Absolute blood flow velocity and orientation are measured by a dual-mode imaging technology using photoacoustic microscopy and optical coherence tomography (PA/OCT). The blood flow velocity perpendicular to the direction of the probe beam is measured by photoacoustic correlation spectroscopy. The blood flow velocity parallel to the direction of the probe beam is measured by the Doppler OCT. Then the absolute velocity and orientation of blood flow are obtained. For the same fluid sample with different tilt angles, the standard deviation of the absolute blood flow velocity which is 1 mm·s -1 measured by PA/OCT is 0.02 mm·s -1 and the correlation coefficient between the blood flow orientation measured by PA/OCT and the actual flow orientation is 0.997. The experimental results show that the PA/OCT is suitable for measuring absolute blood flow velocity and orientation.