We report on the observation of the highly forbidden S10–P30 optical clock transition in laser-cooled Hg199 atoms. More than 95% depletion of cold Hg199 atoms is detected in the magneto-optical trap. Using the free-of-field detection method, the AC Stark shift from the cooling laser is removed from the in-field spectroscopy. At low-power clock laser pumping, the linewidth of the clock spectroscopy is approximately 450 kHz (full width at half-maximum), which corresponds to a Doppler broadening at the atom temperature of 60 μK. We determine the S10–P30 transition frequency to be 1,128,575,290.819(14) MHz by referencing with a hydrogen maser and measuring with a fiber optical frequency comb. Moreover, a weak Doppler-free signal is observed.