光散射学报, 2018, 30 (2): 107, 网络出版: 2018-08-04  

基于SERS的一种快速、有效的挥发物筛选式葱属植物鉴别分类研究

A New SERS-Based Method of Screening Volatiles for Researching the Classification of Allium
作者单位
楚雄师范学院 云南省高校分子光谱重点实验室,云南 楚雄 675000
摘要
本文应用表面增强拉曼散射技术以纳米银溶胶作为基底直接对17种葱属植物的挥发性物质进行了检测,进一步用SERS谱图结合化学计量学多变量统计分析,对17种葱属植物进行鉴别分类研究,并提出一种基于SERS的快速、有效的挥发性物质筛选式葱属植物鉴别分类研究方法。对不同年份制作的纳米银溶胶进行了重现性测试,结果显示纳米银溶胶作为SERS基底对葱属植物的挥发性物质检测重现效果较好;对同一植物不同部位的挥发性物质进行检测,结果显示光谱峰位变化不大,只是个别峰的相对强度发生了变化;对17种葱属植物的挥发性物质进行检测,结果显示:17种葱属植物的挥发物的SERS光谱可分为三组,1-丙硫醇增强组、烯丙基甲基硫醚增强组、二烯丙基二硫增强组,说明纳米银溶胶对葱属植物的挥发物具有选择性增强效果;17种葱属植物挥发物的SERS谱结合聚类分析、因子分析、判别分析进行多变量统计分析,分析结果显示,样品能按三个不同增强组进行准确分类。实验结果表明,基于SERS的挥发物筛选式葱属植物鉴别分类研究方法可以为葱属植物分类研究提供参考信息。
Abstract
This work put forward a fast,effective and SERS-based volatiles screening method for identification and classification of 17 Allium species.Nano-silver colloid was used as substrate to detect volatiles surface-enhanced Raman scattering (SERS) spectra of different parts of the same species.Results showed that peaks positions of their SERS spectra were essentially the same,only the relative intensity of some individual peaks changed;the repeatability of silver colloid produced in different years was very well.Then,volatiles SERS spectra of 17 Allium species were detected,their SERS spectra can be divided into three groups:1-Propanethiol enhanced group,Diallyl disulfide enhanced group and Allyl methyl sulfide enhanced group.The multivariate statistical analysis was performed on the volatiles SERS spectra of 17 Allium species and results confirmed that the 17 species were divided into the same three groups as mentioned above.All above results conclude that SERS-based method of screening volatiles can provide new reference information for discriminating Allium.

张川云, 李伦, 张德清, 司民真. 基于SERS的一种快速、有效的挥发物筛选式葱属植物鉴别分类研究[J]. 光散射学报, 2018, 30(2): 107. ZHANG Chuanyun, LI Lun, ZHANG Deqing, SI Minzhen. A New SERS-Based Method of Screening Volatiles for Researching the Classification of Allium[J]. The Journal of Light Scattering, 2018, 30(2): 107.

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