目的: 建立商陆离体再生体系。方法: 选取商陆的幼茎、茎节、叶片、叶柄和顶芽为外植体,以MS作为基本培养基,通过添加不同浓度配比的植物生长调节剂分别进行愈伤组织、丛生芽和生根诱导,筛选商陆离体再生体系方案。结果: 顶芽和幼茎为外植体诱导的愈伤组织出愈时间早,愈伤组织质量高,以培养基MS+6-BA 0.5 mg/L+2.4-D 0.5 mg/L的诱导率最高,达到100%；其中,只有以顶芽产生的愈伤组织才能分化出丛生芽,芽分化培养基为MS+6-BA 2.0 mg/L+NAA 0.25 mg/L,诱导率为98%；诱导生根的适宜培养基为1/2 MS+NAA 0.3 mg/L,诱导率达100%。结论: 建立和完善了商陆离体再生体系方案,为商陆遗传转化体系的构建奠定了基础。

Objective: The aim is to establish regeneration system in vitro of Phytolacca acinosa Roxb. Methods: To screen regeneration system scheme of Phytolacca acinosa Roxb, young stem and stem node and leaves and petioles and apical buds of Phytolacca acinosa Roxb were chosen as explants, and MS was used as basic medium with different plant growth regulators combinations to induce callus and buds and roots. Results: Callus could be induced from apical buds and young stem with early emergence and high quality, and the callus induction rate reached 100% in medium MS+6-BA 0.5 mg/L+ 2.4-D 0.5 mg/L. Among them, only the callus produced from apical bud could differentiate cluster buds. The medium for bud differentiation was MS+6-BA 2.0 mg/L+NAA 0.25 mg/L, and the induction rate reached 98%. The suitable medium for rooting was 1/2 MS + NAA 0.3 mg/L with 100% induction rate. Conclusion: The regeneration system in vitro of Phytolacca acinosa Roxb was established and perfected, which laid a foundation for the construction of genetic transformation system in Phytolacca acinosa Roxb.