ing at the insufficient attention to influence of plant chlorophyll fluorescence on the accuracy of CO₂ inversion, we investigate the global vegetation fluorescence distribution, and simulate the influence of fluorescence column-averaged CO₂ dry-air mixing ratio (XCO₂). The simulation shows that when chlorophyll fluorescence is neglected, the inversion maximum error of XCO₂ can reach 15×10 ^-6. This bias can be controlled within 0.5×10 ^-6 by synchronous inversion fluorescence in a full-physics based retrieval. We retrieve the summer data of the Greenhouse Gases Observing Satellite (GOSAT) near Park Falls TCCON (The Total Carbon Column Observing Network) site. It is found that the error is corrected from 6×10 ^-6 to less than 2×10 ^-6 based on synchronous inversion fluorescence. This research shows that chlorophyll fluorescence cannot be neglected in high precision CO₂ inversion.