首页 > 论文 > 中国激光 > 45卷 > 12期(pp:1207001--1)

基于并行波前校正算法的大视场深穿透光学显微成像

Large Field-of-View and Deep Tissue Optical Micro-Imaging Based on Parallel Wavefront Correction Algorithm

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

针对较厚的组织,普遍采用的自适应光学技术由于其单次校正视场范围有限,空间光调制器或可变形镜的刷新率有限,难以满足大视场范围波前畸变的快速校正,进而难以满足大视场高速成像的需求。结合共轭型自适应光学系统和相干光自适应校正技术,提出了一种并行的波前畸变校正算法,该算法可以在不增加空间光调制器等刷新次数的前提下,通过并行测量多个导引星的波前畸变,实现大视场范围内像差的一次性校正,为生物组织深处的高速、高分辨成像提供一种可行的参考方案。仿真结果表明:在采用9个导引星时,针对5层随机相位屏构成的薄散射介质,该算法单次校正的有效视场约为传统算法的4.7倍;对于120 μm厚的小鼠大脑组织切片样本,单次校正的有效视场约为传统算法的4.6倍。所提算法可以通过增加导引星的数量来进一步增大一次校正的视场范围,并且不会显著增加校正时间,在活体生物样本的大视场成像中具有广阔的应用前景。

Abstract

As for deep tissues, the field of view of a single correction in the widely used adaptive optics is limited and the refresh rate of a spatial light modulator or a deformable mirror is also limited. Therefore it is difficult for them to satisfy the requirement of large field-of-view (FOV) rapid correction of wavefront distortion and thus that of high-speed imaging. A parallel wavefront correction method is proposed based on the conjugate adaptive optical correction system and the coherent optical adaptive technique. In this method, without increasing the number of refresh times of spatial light modulator, the large FOV of one-time correction can be realized by means of the parallel measurement of wavefront distortion of multiple guide stars, which provides a feasible reference solution for the high-speed and high-resolution imaging of deep tissues. The simulation results show that when 9 guiding stars are used, the effective FOV of a single correction by the proposed method is about 4.7 times than that of the conventional method for a thin scattering medium composed of 5 layers of random phase masks, and 4.6 times than that of the conventional method for 120-μm thick mouse brain tissue. Moreover, the proposed method can further improve the FOV of one-time correction by increasing the number of guide stars while the correction time does not significantly increase, which has broad application prospect in the large FOV imaging of in vivo biological tissues.

Newport宣传-MKS新实验室计划
补充资料

中图分类号:O439

DOI:10.3788/cjl201845.1207001

基金项目:国家自然科学基金(31571110,81771877)、浙江省自然科学基金(LY16F050002,LZ17F050001)

收稿日期:2018-07-04

修改稿日期:2018-07-26

网络出版日期:2018-08-05

作者单位    点击查看

赵琪:浙江大学光电科学与工程学院,现代光学仪器国家重点实验室, 浙江 杭州 310027
石鑫:浙江大学光电科学与工程学院,现代光学仪器国家重点实验室, 浙江 杭州 310027
龚薇:浙江大学医学院神经科学研究中心, 浙江 杭州 310058
胡乐佳:浙江大学光电科学与工程学院,现代光学仪器国家重点实验室, 浙江 杭州 310027
郑瑶:浙江大学光电科学与工程学院,现代光学仪器国家重点实验室, 浙江 杭州 310027
祝欣培:浙江大学医学院神经科学研究中心, 浙江 杭州 310058
斯科:浙江大学光电科学与工程学院,现代光学仪器国家重点实验室, 浙江 杭州 310027浙江大学医学院神经科学研究中心, 浙江 杭州 310058

联系人作者:斯科(kesi@zju.edu.cn)

【1】Betzig E, Trautman J K. Near-field optics: microscopy, spectroscopy, and surface modification beyond the diffraction limit[J]. Science, 1992, 257(5067): 189-195.

【2】Helmchen F, Denk W. Deep tissue two-photon microscopy[J]. Nature Methods, 2005, 2(12): 932-940.

【3】Ahrens M B, Orger M B, Robson D N, et al. Whole-brain functional imaging at cellular resolution using light-sheet microscopy[J]. Nature Methods, 2013, 10(5): 413-420.

【4】Babcock H W.The possibility of compensating astronomical seeing[J]. Publications of the Astronomical Society of the Pacific, 1953, 65(386): 229.

【5】Rao C H, Jiang W H, Ling N,et al. Temporal correction effectiveness of adaptive optical system for light wave atmospheric propagation[J]. Acta Optica Sinica, 2001, 21(8): 933-938.
饶长辉, 姜文汉, 凌宁, 等. 自适应光学系统对实际大气湍流波前的时域校正效果[J]. 光学学报, 2001, 21(8): 933-938.

【6】Jiang W H, Zhang Y D, Rao C H, et al. Progress on adaptive optics of institute of optics and electronics, Chinese Academy of Sciences[J]. Acta Optica Sinica, 2011, 31(9): 0900106.
姜文汉, 张雨东, 饶长辉, 等. 中国科学院光电技术研究所的自适应光学研究进展[J]. 光学学报, 2011, 31(9): 0900106.

【7】Liang J Z, Williams D R, Miller D T. Supernormal vision and high-resolution retinal imaging through adaptive optics[J]. Journal of the Optical Society of America A, 1997, 14(11): 2884-2892.

【8】Tan Z J, Xie J, Lu J, et al. High spatial resolution confocal microscopy using adaptive optics[J]. Laser & Optoelectronics Progress, 2012, 49(9): 090002.
谭佐军, 谢静, 卢军, 等. 自适应光学高分辨率共聚焦显微成像技术[J]. 激光与光电子学进展, 2012, 49(9): 090002.

【9】Booth M J. Adaptive optical microscopy: the ongoing quest for a perfect image[J]. Light: Science & Applications, 2014, 3(4): e165.

【10】Wang C, Ji N. Characterization and improvement of three-dimensional imaging performance of GRIN-lens-based two-photon fluorescence endomicroscopes with adaptive optics[J]. Optics Express, 2013, 21(22): 27142-27154.

【11】Wang K, Milkie D E, Saxena A, et al. Rapid adaptive optical recovery of optimal resolution over large volumes[J]. Nature Methods, 2014, 11(6): 625-628.

【12】Wang C, Liu R, Milkie D E, et al. Multiplexed aberration measurement for deep tissue imaging in vivo[J]. Nature Methods, 2014, 11(10): 1037-1040.

【13】Li J, Beaulieu D R, Paudel H, et al. Conjugate adaptive optics in widefield microscopy with an extended-source wavefront sensor[J]. Optica, 2015, 2(8): 682-688.

【14】Mertz J, Paudel H, Bifano T G. Field of view advantage of conjugate adaptive optics in microscopy applications[J]. Applied Optics, 2015, 54(11): 3498-3506.

【15】Park J H, Sun W, Cui M. High-resolution in vivo imaging of mouse brain through the intact skull[J]. Proceedings of the National Academy of Sciences, 2015, 112(30): 9236-9241.

【16】Wu T W, Cui M. Numerical study of multi-conjugate large areawavefront correction for deep tissue microscopy[J]. Optics Express, 2015, 23(6): 7463-7470.

【17】Cui M. Parallel wavefront optimization method for focusing light through random scattering media[J]. Optics Letters, 2011, 36(6): 870-872.

【18】Liu R, Milkie D E, Kerlin A, et al. Direct phase measurement in zonal wavefront reconstruction using multidither coherent optical adaptive technique[J]. Optics Express, 2014, 22(2): 1619-1628.

【19】Si K, Fiolka R, Cui M. Fluorescence imaging beyond the ballistic regime by ultrasound-pulse-guided digital phase conjugation[J]. Nature Photonics, 2012, 6(10): 657-661.

【20】Si K, Reto F, Cui M. Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy[J]. Scientific Reports, 2012, 2(10): 748.

引用该论文

Zhao Qi,Shi Xin,Gong Wei,Hu Lejia,Zheng Yao,Zhu Xinpei,Si Ke. Large Field-of-View and Deep Tissue Optical Micro-Imaging Based on Parallel Wavefront Correction Algorithm[J]. Chinese Journal of Lasers, 2018, 45(12): 1207001

赵琪,石鑫,龚薇,胡乐佳,郑瑶,祝欣培,斯科. 基于并行波前校正算法的大视场深穿透光学显微成像[J]. 中国激光, 2018, 45(12): 1207001

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF