激光与光电子学进展, 2018, 55 (12): 120901, 网络出版: 2019-08-01  

定量干涉显微流式细胞仪的研究与设计 下载: 1134次

Research and Design of Quantitative Interferometric Microscopic Cytometer
作者单位
1 西安工业大学电子信息工程学院, 陕西 西安 710032
2 上海电力学院电子与信息工程学院, 上海 200090
3 中国工程物理研究院流体物理研究所, 四川 绵阳 621900
4 江南大学理学院光电信息科学与工程系, 江苏 无锡 214122
摘要
生物细胞的成像是生物和物理领域中的重要研究方向,尤其是大量细胞的测量与分析对于生物研究、疾病诊断具有重要的研究价值和意义。传统的显微检测仅局限于细胞形状的定性观察,且受视场限制,观察的细胞数量难以达到统计要求。将定量干涉显微和视场扫描结合,设计并实现了用于大量细胞成像与分析的定量干涉显微流式细胞仪。定量干涉显微可以恢复细胞的相位分布,结合视场扫描可以对大量细胞信息进行获取与分析。分别选用扩展主程序分析算法、正则化光学流场算法和傅里叶相位恢复算法,并结合不同的扫描方式,实现了对大量细胞相位面积、相位体积和圆率等不同参数的测量与统计。该系统有望在大通量高速细胞检测中获得应用。
Abstract
Bio-cell imaging is one of the important research directions in the biological and physical fields, especially the measurement and analysis of large amount of cells have major research value and significance in the bio-research and disease-diagnosis. However, the traditional microscopic detection is only restricted to the qualitative observation of cellular contours. Moreover, the amount of measurement samples is difficult to satisfy the statistical requirements due to the limitation of field of view. Thus a quantitative interferometric microscopic cytometer is designed based on the combination of quantitative interferometric microscopy and field of view scanning, and is used for the imaging and analysis of large amount of cells, in which the quantitative interferometric microscopy is capable of retrieving the cellular phase distribution, and of obtaining and analyzing a large amount of cellular information by combining the field of view scanning. The expanded principle component analysis algorithm, regularized optical flowing algorithm and Fourier phase retrieval algorithm are used, respectively. In addition, the different scanning modes are also combined, and thus the measurements and statistics of phase area, phase volume, circle ratio and other parameters of cells are obtained. The whole system is expected to obtain applications in high-throughput and high-speed cellular detection.

闫克丁, 薛亮, 黄华川, 王绶玙. 定量干涉显微流式细胞仪的研究与设计[J]. 激光与光电子学进展, 2018, 55(12): 120901. Keding Yan, Liang Xue, Huachuan Huang, Shouyu Wang. Research and Design of Quantitative Interferometric Microscopic Cytometer[J]. Laser & Optoelectronics Progress, 2018, 55(12): 120901.

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