多光子成像技术是一种层析能力好、信噪比高的新型光学成像技术。在皮肤光学三维检测中, 多光子技术已经应用于无创在体成像, 且已得到产业化开发。本文将首先介绍多光子皮肤检测系统的若干核心技术, 即双光子自发荧光技术、二次谐波成像技术、荧光寿命成像技术、相干反斯托克斯-拉曼成像技术等, 然后简要介绍多光子成像系统在皮肤疾病成像检测上的应用, 最后分析该系统的优势和未来可能的发展趋势。

Multi-photon imaging is a new technique for optical imaging with inherent optical sectioning abilities and a high signal-to-noise ratio. Multi-photon microscopy has applications in dermatological imaging for its non-invasive and in situ imaging capability. As such, it has been applied commercially. In this paper, we first introduce the core technologies of multiphoton microscopy including two-photon auto-fluorescence imaging, second-harmonic generation(SHG), fluorescence life time imaging(FLIM) and coherent anti-Stokes Raman scattering(CARS). We then introduce its application on skin imaging analysis. Finally, we analyze the advantages and the possible future development of this technology.