本研究利用光学相干层析术OCT对泼尼松龙诱导的斑马鱼骨质疏松模型进行活体成像，并结合电镜能谱技术定量分析斑马鱼模型骨质的钙磷元素含量及分布情况，共同探讨OCT方法在基于斑马鱼模型开展的骨质疏松研究中的使用价值。选取40条3月龄野生型斑马鱼暴露于50 μmol/L泼尼松龙溶液和含0.5%DMSO的溶液中(对照组)，28.5 ℃下培养，分别于第5、10、20天取出浸药组和对照组进行OCT活体成像，比较两者光散射特征。在每个时间点的成像之后，将浸药组的5条斑马鱼处死，然后取颅骨进行元素含量电镜扫描能谱分析。本研究利用50 μmol/L泼尼松龙溶液培养斑马鱼至第20天，成功构建了斑马鱼骨质疏松模型。与对照组相比，模型组活体OCT成像显示骨组织光散射减弱，光子量明显减少，呈不均匀分布。能谱元素检查结果说明颅骨内所含钙、磷比例明显下降，证实骨质疏松发生，骨量减少。OCT成像方法在对斑马鱼骨质疏松模型进行活体、实时、无创等研究方面具有重要价值，本试验也为骨质疏松疾病的研究和药物筛选等方面提供了新的有效的方法。

This study aim to explore the application value of OCT(optical coherence tomography) in the study of osteoporosis model of zebrafish. In vivo evaluation of prednisolone-induced osteoporosis of the adult zebrafish was conducted with optical coherence tomography, and the components of the model’s bone were measured by scanning electron microscopy. A total of 40 adult wild type zebrafish (90 day) were selected for this study. Out of 40 fish, 30 were exposed to 50 μmol/L prednisolone solution, and 10 were set up with 0.5% DMSO medium(ascertained as controls). The zebrafish were kept in our flowthrough aquaria at a temperature of 28 ℃. The imaging of the bone structure using high resolution optical coherence tomography were performed at days 0, 7, 14 or 21 after the prednisolone were induced, comparing their light scattering characteristics. At each time point after OCT imaging experiment, the skull of zebrafish was collected for elemental content analysis by electron microscopy. The osteoporosis model using zebrafish induced by 50 μmol/L prednisolone solution was successfully developed for 20 days. Compared with the control group, the living OCT imaging in the model group showed that the light scattering of the skull tissue was weakened and the photon quantity was significantly reduced, showing an uneven distribution. The results of energy spectrum element examination demonstrated that, the proportion of calcium and phosphorus in bone decreased significantly, indicating osteoporosis and bone loss. The results demonstrated that OCT is an excellent technology in evaluating in the study of the osteoporosis model of zebrafish in vivo, real-time and non-invasive.This experiment also provides a new and effective method for the study of osteoporosis disease and drug discovey.