中国激光, 2019, 46 (4): 0407001, 网络出版: 2019-05-09
光片荧光显微镜长时间的稳定成像 下载: 1334次
Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope
成像系统 光片荧光显微镜 机械偏移 4f系统 补偿 最优图像 imaging systems light sheet fluorescence microscopy mechanical drift 4f optical setup compensation optimal image
摘要
利用4f (f 为焦距)系统和电子耦合器件相机跟踪样品的位置,通过纳米平移台对机械漂移进行了补偿,使样品始终处于光片的束腰位置,从而获得了最优图像。所提方法可以对几纳米的机械偏移进行补偿。使用自主搭建的光片荧光显微镜对直径为几微米的荧光微球进行了长时间成像,验证了所提方法的可行性。
Abstract
By using a 4f (f is the focal length) optical setup and a charge coupled device camera, the sample position is tracked. The mechanical drift is compensated by the nano-translation table which ensures the sample always at the beam waist of the light sheet and thus an optimal image is obtained. With the proposed method, the mechanical drift of several nanometers can be compensated. By using the home-made light sheet fluorescence microscopy, the long-time imaging of fluorescent microspheres with diameters of several micrometers is acquired, which confirms the feasibility of the proposed method.
张球, 梁东, 白丽华, 刘军. 光片荧光显微镜长时间的稳定成像[J]. 中国激光, 2019, 46(4): 0407001. Qiu Zhang, Dong Liang, Lihua Bai, Jun Liu. Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope[J]. Chinese Journal of Lasers, 2019, 46(4): 0407001.