Journal of Innovative Optical Health Sciences, 2019, 12 (2): 1950004, Published Online: Apr. 16, 2019  

Optimization method to suppress background for imaging multiple planes

Vannhu Le 1,2,3,*
Author Affiliations
1 Department of Optical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet Street, Tu Liem District, Hanoi, Vietnam
2 State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
3 Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
Abstract
3D live imaging is important for better understanding of biological processes. To obtain biological dynamic process, high imaging speed is required. In order to improve speed in 3D live imaging, simultaneous imaging of multiple planes throughout a 3D volume has been proposed. However, a main disadvantage of this method is the cross-talk from neighboring imaging planes. In this paper, we propose an optimization method to suppress background from neighboring imaging planes. A D-aperture is used to generate multiple light sheets. An optimization method to suppress background is presented. The simulation results demonstrated that the proposed method can be used to suppress the effectiveness of background from neighboring light sheets.

Vannhu Le. Optimization method to suppress background for imaging multiple planes[J]. Journal of Innovative Optical Health Sciences, 2019, 12(2): 1950004.

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