光谱学与光谱分析, 2020, 40 (7): 2005, 网络出版: 2020-12-04   

应用SFS与FTIR二维相关光谱研究水体中DOM组成结构及空间分异特征

Applying SFS, FTIR and 2D-COS to Analyze Structural Compositions of DOM in Aquatic System, and Reveal Their Special Variation
作者单位
1 成都信息工程大学资源环境学院, 四川 成都 610225
2 中国环境科学研究院流域水环境污染综合治理研究中心, 北京 100012
摘要
二维相关光谱(2D-COS)是将动态光谱扩展到两个维度, 包含同谱与异谱, 具有扩展分离重叠峰、 判断不同组分变化顺序等特征。 2D-COS异谱将两种不同类型的光谱整合成为一种光谱, 研究不同波段之间的相关性, 辨析组分变化的两种光谱的互补关系。 应用同步荧光(SFS)、 傅里叶变换红外(FTIR)与2D-COS方法, 研究乌梁素海水体中DOM组成结构特征, 分析DOM组分及官能团之间的协变性, 揭示其空间分异规律。 水体中DOM由CO, C—H, N—H, C—O等官能团构成, 主要包含类蛋白质 (PLF)、 微生物类腐殖质(MHLF)、 类富里酸(FLF)和类胡敏酸(HLF)等组分, 其中MHLF为主要组分。 水体中荧光物质、 PLF以及MHLF的相对丰度由大到小顺序均为北部>南部>中部。 FLF相对丰度由大到小的顺序是南部>北部>中部, 而HLF相对丰度较稳定。 SFS的2D-COS分析表明: 北部水体中PLF变动较大, 变动顺序为PLF→MHLF; 中部水体中MHLF变动较大, 其与PLF变动趋势相反, 变动顺序为PLF→MHLF; 南部水体中FLF变化较大, 变动顺序为PLF→FLF→MHLF。 FTIR的2D-COS分析表明: 北部水体中C—O变动较大, 与C—H, CO变动趋势相同, 变化顺序为C—O→C—H→CO; 中部水体中C—O变动较大, 与N—H, CO变动趋势相同, 变动顺序为C—O→N—H→C—H→CO; 南部水体中C—O变动较大, 与N—H, CO变动趋势相同, 变动顺序为C—O→C—H→CO。 SFS与FTIR的2D-COS异谱相关分析表明: 北部水体中PLF与C—O的变化趋势相同, 中部水体中C—O, N—H, CO与MHLF变化趋势相同, 而与PLF的变化趋势相反, 南部水体中C—O, C—H, N—H, CO与FLF变化趋势相同。
Abstract
Two-dimensional correlation spectroscopy (2D-COS) can extend the dynamic spectrum to two dimensions, which contains synchronous and asynchronous maps. It not only can separate overlapping peaks to enhance resolution, but also discriminate variation orders of different components. 2D-COS heterospectrum can combine two different spectra into one spectrum, which is applied to trace the relationship between different bands, and identify complementarityof component variations. Synchrotron fluorescence spectroscopy (SFS), Fourier transform infrared (FTIR) and two-dimensional correlation spectroscopy (2D-COS) were applied to analyze the structural composition of dissolved organic matter (DOM) in Wuliangsuhai Lake, trace the covariance between DOM components and functional groups, and reveal their spatial variations. According to the FTIR spectroscopy, DOM was mainly composed of CO, C—H, N—H, C—O functional groups. DOM contained protein-like (PLF), microbial humic-like (MHLF), fulvic-like (FLF) and humic-like (HLF) components by the SFS, among which the MHLF was the dominated component. The decreasing order of the contents of fluorescent materials, PLF and MHLF abundance was north>south>central. The decreasing of the relative FLF abundance was south>north>central, but the relative abundance of HLF kept stable. According to the SFS 2D-COS, the PLF variation was larger than the MHLF in the northern region, whose order was PLF→MHLF; the MHLF showed the negative correlation with the PLF in the central region, whose variation order was PLF→MHLF; the FLF variation was larger than the PLF and MHLF in the southern region, whose order was PLF→FLF→MHLF. According to the FTIR 2D-COS, the C—O was positively correlated to the C—H and CO in the northern region, whose variation order was C—O→C—H→CO; the C—O exhibited the positive correlation with the N—H and CO in the central region, whose variation order was C—O→N—H→C—H→CO; the C—O was the positive relationship with the N—H and CO in the southern region, whose variation order was C—O→C—H→CO. According to the 2D-COS between SFS and FTIR, the C—O showed the positive correlation with the PLF in the northern region; the C—O, N—H and CO had the positive correlations with the MHLF in the central region, but had the negative correlations with the PLF; the C—O, C—H, N—H and CO exhibited thepositive relationship with the FLF. In summary, SFS and FTIR combined with 2D-COS can be used as an effective method to analyze the covariance of DOM components and functional groups in water and reveal their spatial variation.

李崇蔚, 于会彬, 杨芳, 郭旭晶, 高红杰, 白杨. 应用SFS与FTIR二维相关光谱研究水体中DOM组成结构及空间分异特征[J]. 光谱学与光谱分析, 2020, 40(7): 2005. LI Chong-wei, YU Hui-bin, YANG Fang, GUO Xu-jing, GAO Hong-jie, BAI Yang. Applying SFS, FTIR and 2D-COS to Analyze Structural Compositions of DOM in Aquatic System, and Reveal Their Special Variation[J]. Spectroscopy and Spectral Analysis, 2020, 40(7): 2005.

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