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利用光谱和时间分辨的多光子显微技术识别人体冠状动脉粥样硬化斑块

Identification of Human Coronary Atherosclerotic Plaques using Spectrum- and Time-Resolved Multiphoton Microscopy

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摘要

采用光谱和时间分辨的多光子显微技术对8例未染色的人体冠状动脉组织进行研究,根据荧光光谱分离出冠状动脉内膜中的弹性纤维和胶原纤维,并计算粥样硬化病变与非病变冠状动脉内膜中两种纤维信号的比值,从而获得胶原纤维和弹性纤维相对含量的变化。采用荧光寿命评估了冠状动脉粥样硬化组织中弹性纤维生化特性的变化,结果显示,冠状动脉硬化斑块区域具有较短的平均荧光寿命。光谱和时间分辨的多光子显微技术能有效识别冠状动脉粥样硬化斑块,有望成为研究冠状动脉粥样硬化病变的新方法。

Abstract

This study evaluated 8 unstained human coronary tissues ex vivo using spectrum- and time-resolved multiphoton microscopy. First, according to the spectra, the elastin fibers and collagen fibers in the coronary arterial intima can be separated clearly. Second, the ratios of the two types of fiber signals were calculated to assess changes in the relative content of the collagen and elastin fibers in the coronary arterial wall caused by an atherosclerotic lesion. Third, we assessed biochemical variations of the elastin fibers in the coronary atherosclerotic tissues by measuring fluorescence lifetime and found that the coronary atherosclerotic plaque has a lower mean fluorescence lifetime than a normal tissue. This study demonstrates that spectrum- and time-resolved multiphoton microscopy can effectively identify coronary atherosclerotic plaques, thus indicating its potential as a novel research tool for studying coronary arteriosclerotic lesions in the future.

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中图分类号:Q631

DOI:10.3788/CJL202047.0207025

所属栏目:生物医学光子学与激光医学

基金项目:国家自然科学基金、深圳市基础研究、广东省科技计划项目;

收稿日期:2019-10-08

修改稿日期:2019-12-02

网络出版日期:2020-02-01

作者单位    点击查看

张荣丽:华南理工大学医学院, 广东 广州 510006华南理工大学附属广东省人民医院, 广东 广州 510080中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
李慧:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
吴岳恒:广东省人民医院,广东省医学科学院,广东省华南结构心脏病省级重点实验室, 广东省心血管病研究所, 广东 广州 510080
余佳:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
刘尚敏:广东省人民医院广东省医学科学院医学研究部, 广东 广州 510080
郑炜:中国科学院深圳先进技术研究院生物医学光学与分子影像研究室, 广东 深圳 518055
林展翼:华南理工大学医学院, 广东 广州 510006华南理工大学附属广东省人民医院, 广东 广州 510080

联系人作者:郑炜(zhengwei@siat.ac.cn); 林展翼(linzhanyi@hotmail.com);

备注:国家自然科学基金、深圳市基础研究、广东省科技计划项目;

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引用该论文

Zhang Rongli,Li Hui,Wu Yueheng,Yu Jia,Liu Shangmin,Zheng Wei,Lin Zhanyi. Identification of Human Coronary Atherosclerotic Plaques using Spectrum- and Time-Resolved Multiphoton Microscopy[J]. Chinese Journal of Lasers, 2020, 47(2): 0207025

张荣丽,李慧,吴岳恒,余佳,刘尚敏,郑炜,林展翼. 利用光谱和时间分辨的多光子显微技术识别人体冠状动脉粥样硬化斑块[J]. 中国激光, 2020, 47(2): 0207025

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