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氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器

An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating

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摘要

提出一种基于氧化石墨烯包裹金纳米壳(EGO-AuNS)修饰长周期光纤光栅(LPFG)的新型免疫传感器,并将其应用于禽流感病毒(AIV)检测。利用静电结合原理将石墨烯(GO)包裹在金纳米壳(AuNS)表面形成EGO-AuNS复合材料;采用硅烷偶联剂以共价键结合方式将其固定在LPFG表面;再以AIV单克隆抗体(AIV-MAbs)为特异性生物分子识别单元,固定于光栅表面构成EGO-AuNS-LPFG免疫传感器。结果表明:在折射率(RI)1.333--1.411范围内,EGO-AuNS-LPFG传感器RI灵敏度高达-66.60 nm/RIU,约为普通LPFG传感器的6倍。通过对不同浓度等级AIV抗原溶液的检测,表明该免疫传感器的检测极限约为8 ng/mL,饱和点约为50 μg/mL,在其线性响应区域的检测灵敏度约为2946.25 pm/(μg·mL -1),约为基于GO涂覆包层腐蚀型普通LPFG的AIV免疫传感器灵敏度的7.3倍;此外,其对AIV分子的解离系数约为3.49×10 -9 mol/L。通过对几种尿囊液的对照检测实验,表明该免疫传感器且具有良好的特异性和临床性有效性,因此它在生物医学领域有较大应用潜力。

Abstract

In this study, a novel immunosensor based on graphene-oxide (GO)-encapsulated Au-nanoshell (EGO-AuNS)-coated long-period fiber grating (LPFG) is presented for avian influenza virus (AIV) detection. The EGO-AuNS composites were obtained by coating GO on the surface of AuNS via electrostatic bonding, and immobilized on the surface of LPFG via covalent bonding using a silane coupling agent. Then, AIV monoclonal antibodies (AIV-MAbs), which serve as specific biomolecule recognition units, were fixed on the grating surface to realize the EGO-AuNS-LPFG immunosensor. The experimental results indicate that the refractive index (RI) sensitivity of the EGO-AuNS-LPFG immunosensor is -66.60 nm/RIU when RI is from 1.333 to 1.411, which is six times greater than that of the LPFG-based sensor. Based on the detection of AIV antigen solutions with different concentrations, the limit of detection of the immunosensor is observed to be approximately 8 ng/mL. Further, the immunosensor becomes saturated at approximately 50 μg/mL and exhibits a linearity sensitivity of approximately 2946.25 pm/(μg·mL -1), which is 7.3 times greater than that of the GO-coated cladding-etched LPFG immunosensor. Based on the detection results of several allantoic fluids, the proposed immunosensor exhibits good specificity and clinical effectiveness. Furthermore, it exhibits considerable promise for application in the biomedical field.

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中图分类号:TN253

DOI:10.3788/AOS202040.1806001

所属栏目:光纤光学与光通信

基金项目:国家自然科学基金、重庆市教育委员会科学技术研究计划青年项目、重庆市自然科学基金面上项目、重庆理工大学研究生创新项目;

收稿日期:2020-04-20

修改稿日期:2020-06-09

网络出版日期:2020-09-01

作者单位    点击查看

石胜辉:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
吴德操:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
王鑫:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
聂青林:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
刘志江:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
罗彬彬:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
刘恩华:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
柳鹏:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054
赵明富:重庆理工大学光纤传感与光电检测重庆市重点实验室, 重庆 400054

联系人作者:罗彬彬(luobinbin@cqut.edu.cn); 赵明富(luobinbin@cqut.edu.cn);

备注:国家自然科学基金、重庆市教育委员会科学技术研究计划青年项目、重庆市自然科学基金面上项目、重庆理工大学研究生创新项目;

【1】Huang X C, Wu X W, Gao S C, et al. Response characteristic of twisting second-azimuthal-order few-mode long-period fiber grating [J]. Chinese Journal of Lasers. 2019, 46(12): 1206001.
黄新成, 吴小文, 高社成, 等. 角向二阶少模长周期光纤光栅的扭转响应特性 [J]. 中国激光. 2019, 46(12): 1206001.

【2】Srinivasan R, Umesh S, Murali S, et al. Bare fiber Bragg grating immunosensor for real-time detection of Escherichia coli bacteria [J]. Journal of Biophotonics. 2017, 10(2): 224-230.

【3】Sridevi S, Vasu K S, Asokan S, et al. Sensitive detection of C-reactive protein using optical fiber Bragg gratings [J]. Biosensors and Bioelectronics. 2015, 65: 251-256.

【4】Pilla P, Sandomenico A, Malachovská V, et al. A protein-based biointerfacing route toward label-free immunoassays with long period gratings in transition mode [J]. Biosensors and Bioelectronics. 2012, 31(1): 486-491.

【5】Sun D D, Guo T, Ran Y, et al. In-situ DNA hybridization detection with a reflective microfiber grating biosensor [J]. Biosensors and Bioelectronics. 2014, 61: 541-546.

【6】DeLisa M P, Zhang Z, Shiloach M, et al. Evanescent wave long-period fiber Bragg grating as an immobilized antibody biosensor [J]. Analytical Chemistry. 2000, 72(13): 2895-2900.

【7】Chiavaioli F, Biswas P, Trono C, et al. Sol-gel-based titania-silica thin film overlay for long period fiber grating-based biosensors [J]. Analytical Chemistry. 2015, 87(24): 12024-12031.

【8】Chiavaioli F, Biswas P, Trono C, et al. Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings [J]. Biosensors and Bioelectronics. 2014, 60: 305-310.

【9】Qi Y F, Jia C, Liu L, et al. Biosensing properties of nanofilm-modified long-period fiber gratings [J]. Acta Optica Sinica. 2018, 38(10): 1006005.
齐跃峰, 贾翠, 刘磊, 等. 纳米膜修饰长周期光纤光栅生物传感特性研究 [J]. 光学学报. 2018, 38(10): 1006005.

【10】He Z H, Tian F, Zhu Y N, et al. Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor [J]. Biosensors and Bioelectronics. 2011, 26(12): 4774-4778.

【11】Malachovská V, Ribaut C, Voisin V, et al. Fiber-optic SPR immunosensors tailored to target epithelial cells through membrane receptors [J]. Analytical Chemistry. 2015, 87(12): 5957-5965.

【12】Luo B B, Xu Y F, Wu S X, et al. A novel immunosensor based on excessively tilted fiber grating coated with gold nanospheres improves the detection limit of Newcastle disease virus [J]. Biosensors and Bioelectronics. 2018, 100: 169-175.

【13】Luo B B, Wu S X, Wang L L, et al. Newcastle disease virus immunosensor based on 81° tilted fiber grating [J]. Acta Optica Sinica. 2017, 37(11): 1106001.
罗彬彬, 吴胜昔, 王玲玲, 等. 基于81°倾斜光纤光栅的新城疫病毒免疫传感器 [J]. 光学学报. 2017, 37(11): 1106001.

【14】Liu C, Cai Q, Xu B J, et al. Graphene oxide functionalized long period grating for ultrasensitive label-free immunosensing [J]. Biosensors and Bioelectronics. 2017, 94: 200-206.

【15】Novoselov K S, Geim A K, Morozov S V, et al. Electric field effect in atomically thin carbon films [J]. Science. 2004, 306: 666-669.

【16】Wang L, Xiong Q R, Xiao F, et al. 2D nanomaterials based electrochemical biosensors for cancer diagnosis [J]. Biosensors and Bioelectronics. 2017, 89: 136-151.

【17】Zhang J, Sun Y, Wu Q, et al. Preparation of graphene oxide-based surface plasmon resonance biosensor with Au bipyramid nanoparticles as sensitivity enhancer [J]. Colloids and Surfaces B: Biointerfaces. 2014, 116: 211-218.

【18】Xu B, Huang J, Ding L Y, et al. Graphene oxide-functionalized long period fiber grating for ultrafast label-free glucose biosensor [J]. Materials Science and Engineering: C. 2020, 107: 110329.

【19】Liu C, Xu B J, Zhou L, et al. Graphene oxide functionalized long period fiber grating forhighly sensitive hemoglobin detection [J]. Sensors and Actuators B: Chemical. 2018, 261: 91-96.

【20】Esposito F, Sansone L, Taddei C, et al. Ultrasensitive biosensor based on long period grating coated with polycarbonate-graphene oxide multilayer [J]. Sensors and Actuators B: Chemical. 2018, 274: 517-526.

【21】Cheng J, Su X O, Wang S, et al. Highly sensitive detection of clenbuterol in animal urine using immunomagnetic bead treatment and surface-enhanced Raman spectroscopy [J]. Scientific Reports. 2016, 6: 32637.

【22】Feng D X, Li L H, Han X W, et al. Simultaneous electrochemical detection of multiple tumor markers using functionalized graphene nanocomposites as non-enzymatic labels [J]. Sensors and Actuators B: Chemical. 2014, 201: 360-368.

【23】Li Q, Wang Q, Yang X H, et al. High sensitivity surface plasmon resonance biosensor for detection of microRNA and small molecule based on graphene oxide-gold nanoparticles composites [J]. Talanta. 2017, 174: 521-526.

【24】Shi S H, Wang X, Luo B B, et al. Avian influenza virus immunosensor based on etched long period fiber grating coated with graphene oxide [J]. Acta Photonica Sinica. 2020, 49(1): 0106002.
石胜辉, 王鑫, 罗彬彬, 等. 氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器 [J]. 光子学报. 2020, 49(1): 0106002.

【25】Vengsarkar A M, Lemaire P J, Judkins J B, et al. Long-period fiber gratings as band-rejection filters [J]. Journal of Lightwave Technology. 1996, 14(1): 58-65.

【26】Sim?es E, Abe I, Oliveira J, et al. Characterization of optical fiber long period grating refractometer with nanocoating [J]. Sensors and Actuators B: Chemical. 2011, 153(2): 335-339.

【27】del Villar I, Matías I R, Arregui F J, et al. Optimization of sensitivity in long period fiber gratings with overlay deposition [J]. Optics Express. 2005, 13(1): 56-69.

【28】Bandyopadhyay S, Biswas P, Chiavaioli F, et al. Long-period fiber grating: a specific design for biosensing applications [J]. Applied Optics. 2017, 56(35): 9846-9853.

【29】Bandyopadhyay S, Shao L Y, Wang C, et al. Study on optimization of nano-coatings for ultra-sensitive biosensors based on long-period fiber grating [J]. Sensing and Bio-Sensing Research. 2020, 27: 100320.

【30】Li B R, Chen C W, Yang W L, et al. Biomolecular recognition with a sensitivity-enhanced nanowire transistor biosensor [J]. Biosensors and Bioelectronics. 2013, 45: 252-259.

【31】Socorro-Leránoz A B, Santano D, del Villar I, et al. Trends in the design of wavelength-based optical fibre biosensors (2008—2018) [J]. Biosensors and Bioelectronics: X. 2019, 1: 100015.

【32】Tam F, Moran C, Halas N. Geometrical parameters controlling sensitivity of nanoshell plasmon resonances to changes in dielectric environment [J]. The Journal of Physical Chemistry B. 2004, 108(45): 17290-17294.

【33】Tang J L, Cheng S F, Hsu W T, et al. Fiber-optic biochemical sensing with a colloidal gold-modified long period fiber grating [J]. Sensors and Actuators B: Chemical. 2006, 119(1): 105-109.

【34】Shi S H, Wang X, Zhao M F, et al. Sensing characteristics of long period fiber grating functionalized with graphite oxide [J]. Optics and Precision Engineering. 2019, 27(11): 2305-2314.
石胜辉, 王鑫, 赵明富, 等. 基于氧化石墨修饰长周期光纤光栅的传感特性 [J]. 光学精密工程. 2019, 27(11): 2305-2314.

【35】Li B B, Xue Q, Li J F, et al. Development of a complex gold-immunochromatography test strip for Newcastle disease virus and avian influenza virus [J]. Animal Husbandry & Veterinary Medicine. 2009, 41(8): 33-37.
李蓓蓓, 薛强, 李锦丰, 等. 新城疫病毒和禽流感病毒复合型胶体金免疫层析试纸条的制备 [J]. 畜牧与兽医. 2009, 41(8): 33-37.

引用该论文

Shi Shenghui,Wu Decao,Wang Xin,Nie Qinglin,Liu Zhijiang,Luo Binbin,Liu Enhua,Liu Peng,Zhao Mingfu. An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating[J]. Acta Optica Sinica, 2020, 40(18): 1806001

石胜辉,吴德操,王鑫,聂青林,刘志江,罗彬彬,刘恩华,柳鹏,赵明富. 氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器[J]. 光学学报, 2020, 40(18): 1806001

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