首页 > 论文 > 中国激光 > 47卷 > 9期(pp:907001--1)

用于OCT成像与pH测量的双模态内窥探头

Dual-Modality Endoscopic Probe for Optical Coherence Tomography Imaging and pH Sensing

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

为了实现生物组织化学特性与物理结构的同时测量,提出了结合光学相干层析扫描(OCT)成像与荧光比率成像的双模态内窥探头。根据OCT成像原理,确定OCT系统的中心波长为1300 nm;根据所选用pH指示剂(SNARF-1)的吸收光谱与反射光谱,确定荧光的激发波长为520 nm。根据ABCD矩阵计算并确定探头的基本结构,基于理论计算结果进行光学元件的加工与探头的组装。此外,搭建了双模态系统,采用该系统测量了探头的横向分辨率与工作距离。通过猪大肠验证了探头同时进行成像和pH检测的能力,实验结果表明:探头能同时进行OCT成像与pH测量,并且具有较高的精度。OCT成像在空气中的横向分辨率约为52 μm,工作距离约为14.6 mm,在生物组织中可以实现精度为0.01的pH测量。

Abstract

To achieve simultaneous measurements of biochemical properties and physical structures, we proposed an endoscopic probe that combines optical coherence tomography (OCT) imaging and fluorescence ratio imaging. Based on the OCT imaging principle, the center wavelength of the OCT system was determined to be 1300 nm. Based on the absorption and reflection spectra of the selected pH indicator, the excitation wavelength of the fluorescence was determined to be 520 nm. Moreover, the basic structure of the probe was calculated and determined according to the ABCD matrix. The processing of the optical element and actual assembly of the probe were performed based on the theoretical calculation results. Furthermore, the dual-modality system was built to measure the transverse resolution and working distance of the probe. The ability of simultaneous imaging and pH detection was demonstrated on pork intestine. The probe can simultaneously perform OCT imaging and pH measurement with high accuracy. The resolution of OCT imaging in air is approximately 52 μm, and the working distance is approximately 14.6 mm. Moreover, the proposed system can measure the pH value of 0.01 units.

广告组1 - 空间光调制器+DMD
补充资料

中图分类号:TN247

DOI:10.3788/CJL202047.0907001

所属栏目:生物医学光子学与激光医学

基金项目:国家自然科学基金、上海市科委产学研医项目;

收稿日期:2020-02-20

修改稿日期:2020-04-26

网络出版日期:2020-09-01

作者单位    点击查看

王健平:上海理工大学医疗器械与食品学院, 上海介入医疗器械工程技术研究中心, 上海 200093
陈明惠:上海理工大学医疗器械与食品学院, 上海介入医疗器械工程技术研究中心, 上海 200093
谈维杰:上海理工大学医疗器械与食品学院, 上海介入医疗器械工程技术研究中心, 上海 200093
冯媛媛:上海理工大学医疗器械与食品学院, 上海介入医疗器械工程技术研究中心, 上海 200093
郑刚:上海理工大学医疗器械与食品学院, 上海介入医疗器械工程技术研究中心, 上海 200093

联系人作者:陈明惠(275296270@qq.com)

备注:国家自然科学基金、上海市科委产学研医项目;

【1】Gora M J, Suter M J, Tearney G J, et al. Endoscopic optical coherence tomography: technologies and clinical applications [J]. Biomedical Optics Express. 2017, 8(5): 2405-2444.

【2】Ding M, Chen Y, Guan W J, et al. Measuring airway remodeling in patients with different COPD staging using endobronchial optical coherence tomography [J]. Chest. 2016, 150(6): 1281-1290.

【3】Bus M T J, Muller B G, de Bruin D M, et al. Volumetric in vivo visualization of upper urinary tract tumors using optical coherence tomography: a pilot study [J]. The Journal of Urology. 2013, 190(6): 2236-2242.

【4】Li P, Yang S S, Ding Z H, et al. Research progress in Fourier domain optical coherence tomography [J]. Chinese Journal of Lasers. 2018, 45(2): 0207011.
李培, 杨姗姗, 丁志华, 等. 傅里叶域光学相干层析成像技术的研究进展 [J]. 中国激光. 2018, 45(2): 0207011.

【5】Shen Y, Chen Z Y, Qiu J R, et al. Research progress on parallel spectral domain optical coherence tomography technology [J]. Chinese Journal of Lasers. 2018, 45(2): 0207004.
沈毅, 陈志彦, 邱建榕, 等. 并行谱域光学相干层析成像技术的研究进展 [J]. 中国激光. 2018, 45(2): 0207004.

【6】Yao W T, Gao W R. Development and application of endoscopic optical coherence tomography [J]. Laser & Optoelectronics Progress. 2018, 55(7): 070002.
姚文涛, 高万荣. 内窥光学层析成像的发展和应用 [J]. 激光与光电子学进展. 2018, 55(7): 070002.

【7】Schartner E P, Henderson M R, Purdey M, et al. Cancer detection in human tissue samples using a fiber-tip pH probe [J]. Cancer Research. 2016, 76(23): 6795-6801.

【8】Musolino S, Schartner E P, Tsiminis G, et al. A portable optical fiber probe for in vivo brain temperature measurements [J]. Proceedings of SPIE. 2016, 1001: 100130Q.

【9】Purdey M S, Thompson J G, Monro T M, et al. Adual sensor for pH and hydrogen peroxide using polymer-coated optical fibre tips [J]. Sensors. 2015, 15(12): 31904-31913.

【10】Cui G H, Jun S B, Jin X, et al. Deep brain optical measurements of cell type-specific neural activity in behaving mice [J]. Nature Protocols. 2014, 9(6): 1213-1228.

【11】Wang X D, Wolfbeis O S. Fiber-optic chemical sensors and biosensors [J]. Analytical Chemistry. 2016, 88(1): 203-227.

【12】Mavadia-Shukla J, Fathi P, Liang W X, et al. High-speed, ultrahigh-resolution distal scanning OCT endoscopy at 800 nm for in vivo imaging of colon tumorigenesis on murine models [J]. Biomedical Optics Express. 2018, 9(8): 3731-3739.

【13】Tsai T H, Potsaid B, Tao Y K, et al. Ultrahigh speed endoscopic optical coherence tomography using micromotor imaging catheter and VCSEL technology [J]. Biomedical Optics Express. 2013, 4(7): 1119-1132.

【14】Luo S T, Wu Y Q, An X, et al. A miniature endoscopic optical coherence tomography probe based on C-lens [J]. IEEE Photonics Journal. 2018, 10(5): 3901310.

【15】Pang Z W, Wu J G. Miniaturized magnetic-driven scanning probe for endoscopic optical coherence tomography [J]. Biomedical Optics Express. 2015, 6(6): 2231-2236.

【16】Lu Y, Li Z L, Nan N, et al. Passively driven probe based on miniaturized propeller for intravascular optical coherence tomography [J]. Scientific Reports. 2018, 8(1): 5150-5157.

【17】Liao W C, Chen T Y, Wang C M, et al. Endoscopic optical coherence tomography with a focus-adjustable probe [J]. Optics Letters. 2017, 42(20): 4040-4043.

【18】Qiu J R, Han T, Liu Z Y, et al. Uniform focusing with an extended depth range and increased working distance for optical coherence tomography by an ultrathin monolith fiber probe [J]. Optics Letters. 2020, 45(4): 976-979.

【19】Ji C K, Feng Y H, Sun L P, et al. Micrometer-resolution in-fiber OCT probe with tunable working distance [J]. Optics Express. 2016, 24(17): 19814-19823.

【20】Song S Y, Li Z L, Gao Y H, et al. Swept source optical coherence tomography system for transdermal drug delivery imaging by microneedles [J]. Chinese Journal of Lasers. 2018, 45(8): 0807001.
宋思雨, 李中梁, 高云华, 等. 用于微针经皮给药成像的扫频OCT系统 [J]. 中国激光. 2018, 45(8): 0807001.

【21】Mazurenka M, Behrendt L, Meinhardt-Wollweber M, et al. Development of a combined OCT-Raman probe for the prospective in vivo clinical melanoma skin cancer screening [J]. Review of Scientific Instruments. 2017, 88(10): 105103.

【22】Li Y, Jing J, Qu Y Q, et al. Fully integrated optical coherence tomography, ultrasound, and indocyanine green-based fluorescence tri-modality system for intravascular imaging [J]. Biomedical Optics Express. 2017, 8(2): 1036-1044.

【23】Woolsey N, Liang C P, Ding Z Y, et al. Surgical navigation probe utilizing optical coherence tomography and laser Doppler . [C]∥Biomedical Optics. Washington, D.C.: OSA. 2014, BT3A: 68.

【24】Xu Y, Wang Y, Qiu J R, et al. IVUS-OCT imaging system for detection of intravascular lipid plaque [J]. Laser & Optoelectronics Progress. 2018, 55(8): 081701.
徐勇, 汪毅, 邱建榕, 等. 面向血管内脂质斑块检测的超声-OCT成像系统 [J]. 激光与光电子学进展. 2018, 55(8): 081701.

【25】Li J W, Schartner E P, Musolino S, et al. Miniaturized single-fiber-based needle probe for combined imaging and sensing in deep tissue [J]. Optics Letters. 2018, 43(8): 1682-1685.

【26】Gatenby R A, Gawlinski E T. A reaction-diffusion model of cancer invasion [J]. Cancer Research. 1996, 56(24): 5745-5753.

【27】Gerweck L E. Tumor pH: implications for treatment and novel drug design [J]. Seminars in Radiation Oncology. 1998, 8(3): 176-182.

【28】Gerweck L E, Seetharaman K. Cellular pH gradient in tumor versus normal tissue: potential exploitation for the treatment of cancer [J]. Cancer Research. 1996, 56(6): 1194-1198.

【29】Kaylor B M, Gatenby R A, Gmitro A F. In vivo measurement of pH in tumor and surrounding tissue using fluorescence ratio imaging . [C]∥Frontiers in Optics. Washington, D.C.: OSA. 2005, FMl1.

【30】Cabioglu N, Hunt K K, Sahin A A, et al. Role for intraoperative margin assessment in patients undergoing breast-conserving surgery [J]. Annals of Surgical Oncology. 2007, 14(4): 1458-1471.

【31】Rouzier R, Extra J M, Carton M, et al. Primary chemotherapy for operable breast cancer: incidence and prognostic significance of ipsilateral breast tumor recurrence after breast-conserving surgery [J]. Journal of Clinical Oncology. 2001, 19(18): 3828-3835.

【32】Elkhuizen P H, Hermans J, et al. Local recurrence after breast-conserving therapy for invasive breast cancer: high incidence in young patients and association with poor survival [J]. International Journal of Radiation Oncology, Biology, Physics. 1998, 40(4): 859-867.

【33】McGuire S. Worldcancer report 2014. Geneva, Switzerland: world health organization, international agency for research on cancer, WHO press, 2015[R] . Advances in Nutrition. 2016, 7(2): 418-419.

【34】Qiu J H, Mei R H. Discussion of ABCD matrix and Gaussian beam optical systems [J]. Optical Instruments. 1991, 13(3): 25-32.
邱锦辉, 梅荣华. ABCD矩阵与高斯光束光学系统的讨论 [J]. 光学仪器. 1991, 13(3): 25-32.

【35】Miao Z P, Liang X Q. Approximation of imaging matrix formula of self-focusing lens Journal of Engineering College of Armed Police Force[J]. 0, 1999(2): 1133-1135.
苗正培, 梁秀清. 自聚焦透镜成像矩阵公式的近似性 武警工程学院学报[J]. 0, 1999(2): 1133-1135.

【36】Chu C S, Su C J. Fluorescence ratiometric optical broad range pH sensor based on CdSe/ZnS quantum dots and O170 embedded in ethyl cellulose matrix [J]. Journal of Lightwave Technology. 2018, 36(4): 857-862.

引用该论文

Wang Jianping,Chen Minghui,Tan Weijie,Feng Yuanyuan,Zheng Gang. Dual-Modality Endoscopic Probe for Optical Coherence Tomography Imaging and pH Sensing[J]. Chinese Journal of Lasers, 2020, 47(9): 0907001

王健平,陈明惠,谈维杰,冯媛媛,郑刚. 用于OCT成像与pH测量的双模态内窥探头[J]. 中国激光, 2020, 47(9): 0907001

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF