Chinese Optics Letters, 2021, 19 (4): 041101, Published Online: Jan. 8, 2021
Enhancement of rapid lifetime determination for time-resolved fluorescence imaging in forensic examination Download: 687次
time-resolved fluorescence imaging fluorescence lifetime image visualization enhancement dual-gated intensity-correlation forensic examination
Abstract
An enhancement method of rapid lifetime determination is proposed for time-resolved fluorescence imaging to discriminate substances with approximate fluorescence lifetime in forensic examination. In the method, an image-exclusive-OR treatment with filter threshold adaptively chosen is presented to extract the region of interest from dual-gated fluorescence intensity images, and then the fluorescence lifetime image is reconstructed based on the rapid lifetime determination algorithm. Furthermore, a maximum and minimum threshold filtering is developed to automatically realize visualization enhancement of the lifetime image. In proof experiments, compared with traditional fluorescence intensity imaging and rapid lifetime determination method, the proposed method automatically distinguishes altered and obliterated documents written by two brands of highlighters with the same color and close fluorescence lifetime.
Xin Zhong, Xinwei Wang, Liang Sun, Yan Zhou. Enhancement of rapid lifetime determination for time-resolved fluorescence imaging in forensic examination[J]. Chinese Optics Letters, 2021, 19(4): 041101.